Monfort J D, Stills H F, Bech-Nielsen S
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210.
J Clin Microbiol. 1988 Nov;26(11):2246-7. doi: 10.1128/jcm.26.11.2246-2247.1988.
Two techniques for the isolation of Campylobacter jejuni from feces, direct plating and thioglycolate broth enrichment, were compared. A total of 272 rectal swab cultures were performed on 156 laboratory dogs. Campylobacter blood agar plates and Campylobacter thioglycolate broth were inoculated immediately upon sampling of the dogs. After incubation at 4 degrees C for 12 to 16 h, material from the Campylobacter thioglycolate medium was inoculated onto Campylobacter blood agar plates. A total of 157 samples were positive for C. jejuni; 154 were positive by the direct method and 112 were positive by the enrichment technique. Forty-five samples which were negative by the enrichment were positive by the direct method, and three samples which were negative by the direct method were positive for C. jejuni by the enrichment method. The use of the enrichment step resulted in an increase in the isolation rate from 56.6 to 57.7%.
比较了两种从粪便中分离空肠弯曲菌的技术，即直接平板接种法和硫乙醇酸盐肉汤增菌法。对156只实验犬进行了总共272次直肠拭子培养。在采集犬的样本后，立即将其接种到弯曲菌血琼脂平板和弯曲菌硫乙醇酸盐肉汤中。在4℃孵育12至16小时后，将硫乙醇酸盐培养基中的物质接种到弯曲菌血琼脂平板上。共有157个样本为空肠弯曲菌阳性；直接法检测出154个阳性样本，增菌技术检测出112个阳性样本。45个经增菌检测为阴性的样本通过直接法检测为阳性，3个经直接法检测为阴性的样本通过增菌法检测为空肠弯曲菌阳性。采用增菌步骤使分离率从56.6%提高到了57.7%。
