Luechtefeld N W, Wang W L, Blaser M J, Reller L B
J Clin Microbiol. 1981 Mar;13(3):438-43. doi: 10.1128/jcm.13.3.438-443.1981.
Immediate culturing of fecal specimens is not always possible, and appropriate methods for transport and storage of Campylobacter fetus subsp. jejuni specimens have not been fully evaluated. Using nine techniques, we studied the survival of C. fetus subsp. jejuni in cecal specimens from infected turkeys. The organisms survived in specimens held without transport medium for 3 to 15 days (median, 9 days) at 4 degrees C, and 2 to 9 days (median, 4 days) at 25 degrees C. Only 20% of specimens frozen for 24 h at either -20 or -70 degrees C yielded C. fetus subsp. jejuni. Specimens dried on filter paper strips were negative for C. fetus subsp. jejuni within 1.5 h. Cary-Blair medium with decreased agar was the best of the six transport media tested, it enabled recovery of the organism from 100% (3 days) and 71% (7 days) of cecal samples held at 4 degrees C and 94% (3 days) and 85% (7 days) of cecal specimens held at 25 degrees C. In contrast, more than half of all cecal specimens held at 4 or 25 degrees C in Culturettes or buffered glycerol saline were negative by 3 days, and all were negative at 7 days. Results with the other three media studied (Campy-thio, thioglycolate medium, and alkaline peptone water) were intermediate. Overnight incubation of specimens in alkaline peptone water at 37 or 42 degrees C did not enhance recovery of C. fetus subsp. jejuni. Therefore, refrigeration without a transport medium is satisfactory for up to 3 days for recovery of C. fetus subsp. jejuni from specimens, however, we recommend the use of Cary-Blair medium with decreased agar for specimens that must be transported or stored for longer than 3 days and for rectal swabs, to prevent drying.
粪便标本的即时培养并非总是可行的，空肠弯曲菌胎儿亚种标本的适当运输和储存方法尚未得到充分评估。我们使用九种技术研究了空肠弯曲菌胎儿亚种在感染火鸡盲肠标本中的存活情况。该菌在4℃无运输培养基保存的标本中存活3至15天（中位数为9天），在25℃存活2至9天（中位数为4天）。在-20℃或-70℃冷冻24小时的标本中，只有20%能培养出空肠弯曲菌胎儿亚种。在滤纸条上干燥的标本在1.5小时内空肠弯曲菌胎儿亚种检测呈阴性。在所测试的六种运输培养基中，琼脂含量降低的 Cary-Blair 培养基效果最佳，在4℃保存的盲肠样本中，该培养基能使100%（3天）和71%（7天）的样本培养出该菌，在25℃保存的盲肠标本中，能使94%（3天）和85%（7天）的样本培养出该菌。相比之下，在 Culturettes 或缓冲甘油盐水中于4℃或25℃保存的所有盲肠标本，超过一半在3天时检测呈阴性，7天时全部呈阴性。所研究的其他三种培养基（Campy-thio、硫乙醇酸盐培养基和碱性蛋白胨水）的结果居中。标本在37℃或42℃的碱性蛋白胨水中过夜培养并不能提高空肠弯曲菌胎儿亚种的回收率。因此，从标本中回收空肠弯曲菌胎儿亚种时，无运输培养基的冷藏在长达3天的时间内是令人满意的，然而，对于必须运输或储存超过3天的标本以及直肠拭子，我们建议使用琼脂含量降低的 Cary-Blair 培养基，以防止干燥。
