Manser P A, Dalziel R W
J Hyg (Lond). 1985 Aug;95(1):15-21. doi: 10.1017/s0022172400062239.
A survey of Campylobacter species in the faeces or rectal contents of domestic animals was carried out using direct and enrichment culture methods. Campylobacters were isolated from 259 (31%) of 846 faecal specimens. The highest isolation rate was found in pigs (66%); lower rates were found in cattle (24%) and sheep (22%). In pigs all the isolates were C. coli, in sheep and cattle about 75% were C. jejuni. Only five isolations of C. fetus subsp. fetus were made, all from cattle. More pigs with diarrhoea had C. coli in their faeces than healthy pigs (77% vs 47%), but such a clear difference in isolation rate between sick and healthy animals was not seen in cattle or sheep. The enrichment method increased the total isolation rate of C. jejuni and C. coli by 33%, but for cattle specimens it increased it by 69% (from 6.5% to 21%). However, the enrichment method failed to detect 16% of positive specimens (mainly C. coli), so direct and enrichment methods should be used for the culture of campylobacters from animal faeces. The results show that cattle, sheep and pigs constitute a large potential source of campylobacter infection for man.
采用直接培养法和富集培养法对家畜粪便或直肠内容物中的弯曲杆菌进行了调查。从846份粪便标本中的259份（31%）分离出了弯曲杆菌。分离率最高的是猪（66%）；牛（24%）和羊（22%）的分离率较低。猪的所有分离株均为大肠弯曲杆菌，羊和牛中约75%为空肠弯曲杆菌。仅从牛中分离出5株胎儿弯曲杆菌胎儿亚种。腹泻猪粪便中大肠弯曲杆菌的检出率高于健康猪（77%对47%），但在牛或羊中未观察到患病动物与健康动物在分离率上有如此明显的差异。富集培养法使空肠弯曲杆菌和大肠弯曲杆菌的总分离率提高了33%，但对牛标本而言，分离率提高了69%（从6.5%提高到21%）。然而，富集培养法未能检测出16%的阳性标本（主要是大肠弯曲杆菌），因此应采用直接培养法和富集培养法对动物粪便中的弯曲杆菌进行培养。结果表明，牛、羊和猪是人类弯曲杆菌感染的一大潜在来源。
