Department of Medical Laboratory SciencesFaculty of Health Sciences, University of Botswana, Gaborone, Botswana.
Botswana-Harvard AIDS Institute Partnership, Gaborone, Botswana.
PLoS One. 2019 Jan 30;14(1):e0211260. doi: 10.1371/journal.pone.0211260. eCollection 2019.
High-risk human papillomavirus (HR-HPV) has been demonstrated to be the necessary cause of cervical carcinoma. High-risk HPV detection has a prognostic significance for the women who are at increased risk of disease progression. HPV genotyping in cervical cancer precursor lesions is crucial for prevention and management of cervical cancer. This study was designed to investigate the distribution of HR-HPV genotypes among a group of patients with high-grade squamous intraepithelial lesions and higher, of the cervix, in Botswana.
185-archived residual formalin-fixed paraffin-embedded cervical biopsies collected between the years, 2006 and 2008 were studied. These tissues were diagnosed with HSIL (n = 146) and squamous cell carcinoma (n = 39). DNA was extracted using the Abbott m2000 analyser (Abbott Laboratories, Illinois) using reagents provided by the manufacturer. HPV genotyping was done using the Abbott RealTime HR-HPV PCR, which qualitatively detects 14 HR-HPV (reported as HPV 16, 18 & Other HR-HPV).
DNA was successfully extracted from 162/185 (87.6%) tissues as indicated by a positive β-globin test. 132/162 (82%) tested positive for HR-HPV The HPV 16 prevalence was 50% (66/132), HPV 18 at 15.2% (20/132) and other Group 1 HR-HPV plus HPV 66 and 68 had a prevalence of 56.1% (74/132). Other HR-HPV types were common in HSIL than in carcinoma, while HPV 16 was more prevalent in carcinomas than other HR-HPV genotypes.
In this study, HPV 16 and other HR-HPV genotypes were commonly associated with HSIL but HPV 18 was uncommon among Botswana women. Our data highlights the need for multivalent HPV vaccines with cross coverage for other high risk HPV other than HPV 16 and 18.
高危型人乳头瘤病毒（HR-HPV）已被证实是宫颈癌的必要病因。高危型 HPV 检测对疾病进展风险增加的女性具有预后意义。在宫颈癌前病变中进行 HPV 基因分型对于宫颈癌的预防和管理至关重要。本研究旨在调查博茨瓦纳一组高级别鳞状上皮内病变及以上宫颈患者中 HR-HPV 基因型的分布。
研究了 2006 年至 2008 年间收集的 185 例存档的福尔马林固定石蜡包埋宫颈活检组织。这些组织被诊断为 HSIL（n=146）和鳞状细胞癌（n=39）。使用 Abbott m2000 分析仪（雅培实验室，伊利诺伊州）和制造商提供的试剂提取 DNA。使用 Abbott RealTime HR-HPV PCR 进行 HPV 基因分型，该方法定性检测 14 种 HR-HPV（报告为 HPV 16、18 和其他 HR-HPV）。
如β-球蛋白试验阳性所示，185 例组织中有 162 例（87.6%）成功提取了 DNA。162 例中有 132 例（82%）HR-HPV 检测呈阳性。HPV 16 的流行率为 50%（66/132），HPV 18 为 15.2%（20/132），其他 1 组 HR-HPV 加上 HPV 66 和 68 的流行率为 56.1%（74/132）。其他 HR-HPV 类型在 HSIL 中比在癌中更为常见，而 HPV 16 在癌中比其他 HR-HPV 基因型更为常见。
在这项研究中，HPV 16 和其他 HR-HPV 基因型通常与 HSIL 相关，但 HPV 18 在博茨瓦纳女性中并不常见。我们的数据强调了需要具有交叉覆盖 HPV 16 和 18 以外其他高危 HPV 的多价 HPV 疫苗。
