Gao L, Zhai R, Zhong Y K, Karthikeyan A, Ren R, Zhang K, Li K, Zhi H J
National Center for Soybean Improvement; Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, P.R. China; National Key Laboratory for Crop Genetics and Germplasm Enhancement; Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Plant Dis. 2015 Apr;99(4):442-446. doi: 10.1094/PDIS-04-14-0405-RE.
Soybean mosaic virus (SMV), belonging to the genus Potyvirus of the family Potyviridae, has a relatively narrow host range almost exclusively confined to leguminous hosts. While disease management through genetic transformation can be an effective approach, soybean remains recalcitrant to routine genetic transformation. In this context, it is important to identify new hosts for SMV that can be used to develop effective transgenic resistance strategies. Transformation in Nicotiana benthamiana is simple and highly efficient; hence, here we demonstrate the infectivity of SMV strain SC7 in N. benthamiana plants. To identify an SMV strain infectious in N. benthamiana, we mechanically inoculated N. benthamiana plants with 37 isolates from 21 (SC1 to SC21) SMV strains. Plants inoculated with isolates of strain SC7 produced mosaic symptoms on leaves. However, N. benthamiana plants inoculated with the 20 other SMV strains showed no visible symptoms. Furthermore, soybean cv. Nannong 1138-2 inoculated with sap prepared from symptomatic N. benthamiana leaves showed typical SMV mosaic symptoms 2 weeks after inoculation. In addition, SMV was detected in symptomatic N. benthamiana and soybean leaves by RT-PCR, DAS-ELISA, and further identified by sequencing. Together, the results indicate that N. benthamiana plants could support multiplication of SMV strain SC7. The findings of this study would be useful for the investigation of SMV resistance using the model plant N. benthamiana.
大豆花叶病毒（SMV）属于马铃薯Y病毒科马铃薯Y病毒属，其寄主范围相对较窄，几乎仅限于豆科寄主。虽然通过基因转化进行病害管理可能是一种有效的方法，但大豆对常规基因转化仍具有抗性。在此背景下，识别可用于开发有效转基因抗性策略的SMV新寄主非常重要。本氏烟草的转化简单且高效；因此，在这里我们证明了SMV株系SC7在本氏烟草植株中的感染性。为了鉴定在本氏烟草中具有感染性的SMV株系，我们用来自21个SMV株系（SC1至SC21）的37个分离物对本氏烟草植株进行了机械接种。接种SC7株系分离物的植株叶片上出现了花叶症状。然而，接种其他20个SMV株系的本氏烟草植株没有出现可见症状。此外，用有症状的本氏烟草叶片制备的汁液接种大豆品种南农1138-2，接种2周后出现典型的SMV花叶症状。另外，通过RT-PCR、双抗夹心ELISA在有症状的本氏烟草和大豆叶片中检测到了SMV，并通过测序进一步鉴定。总之，结果表明本氏烟草植株能够支持SMV株系SC7的增殖。本研究结果将有助于利用模式植物本氏烟草研究SMV抗性。
