Soybean Research Institute, Nanjing Agricultural University, Nanjing 210095, China.
MOA National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2021 Oct 20;22(21):11329. doi: 10.3390/ijms222111329.
TGA transcription factors (TFs) exhibit basal resistance in , but susceptibility to a pathogen attack in tomatoes; however, their roles in soybean () to (SMV) are unknown. In this study, 27 genes were isolated from a SMV hyper-susceptible soybean , designated ~, which were clustered into seven phylogenetic groups. The expression profiles of showed that the highly expressed genes were mainly in Groups I, II, and VII under non-induction conditions, while out of the 27 , 19 responded to SMV-induction. Interestingly, in further transient -SMV pathosystem assay, all the 19 overexpressed did not promote SMV infection in inoculated leaves, but they exhibited basal resistance except one without function. Among the 18 functional ones, GmTGA8 and GmTGA19, with similar motif distribution, nuclear localization sequence and interaction proteins, showed a rapid response to SMV infection and performed better than the others in inhibiting SMV multiplication. This finding suggested that GmTGA TFs may support basal resistance to SMV even from a hyper-susceptible source. What the mechanism of the genes (, , etc.) with basal resistance to SMV is and what their potential for the future improvement of resistance to SMV in soybeans is, are to be explored.
TGA 转录因子（TFs）在番茄中表现出基础抗性，但易受病原体攻击；然而，它们在大豆（）对（SMV）的作用尚不清楚。在这项研究中，从一种对 SMV 高度敏感的大豆中分离出 27 个基因，命名为 ~，它们被聚类为七个系统发育组。的表达谱表明，在非诱导条件下，高表达基因主要存在于第 I、II 和 VII 组，而在 27 个中，有 19 个对 SMV 诱导有反应。有趣的是，在进一步的瞬时 -SMV 病理系统测定中，所有 19 个过表达的基因都没有促进接种叶片中的 SMV 感染，但除了一个没有功能的基因外，它们都表现出基础抗性。在 18 个有功能的基因中，GmTGA8 和 GmTGA19 具有相似的基序分布、核定位序列和相互作用蛋白，对 SMV 感染表现出快速反应，在抑制 SMV 增殖方面表现优于其他基因。这一发现表明，GmTGA TF 可能支持大豆对 SMV 的基础抗性，即使是来自高度敏感的来源。这些具有 SMV 基础抗性的基因（、等）的机制是什么，以及它们在未来提高大豆对 SMV 抗性方面的潜力是什么，都需要进一步探索。
