SAINBIOSE, INSERM U1059, University of Lyon, Saint-Etienne, France.
Laboratory of Immunology and Immunomonitoring, University Hospital of Saint-Etienne, Saint-Etienne, France.
Ann Rheum Dis. 2019 May;78(5):594-599. doi: 10.1136/annrheumdis-2018-213697. Epub 2019 Jan 30.
Association between periodontal disease (PD) and rheumatoid arthritis (RA) has been extensively described, but direct evidence of causal involvement of PD in RA is missing. We investigated the priming role of oral () in PD and subsequent RA and we assessed biomarkers of bone resorption and arthritis development in rats.
Lewis rats were orally exposed to either , or control gel for 1 month and then followed for 8 months. The onset and development of PD was assessed by serology, gingivitis severity and micro-CT (µCT). We investigated arthritis development using circulating proinflammatory markers, anticyclic citrullinated peptide (CCP), anticitrullinated protein antibody (ACPA), ankle histology and µCT.
PD was only observed in the treated rats, as early as 1 month postexposure. Joint and systemic inflammation were detected only in the group after 4 and 8 months. At 8 months, inflammatory cell infiltrate was observed in ankle joints and paralleled cortical erosions and overall cortical bone reduction. Furthermore, anti-CCP2 correlated with local and systemic bone loss.
In our long-term study, PD induced by oral exposure to triggered seropositive arthritis, with systemic inflammation and bone erosions. This is the first in vivo demonstration of arthritis induced by oral priming with .
牙周病（PD）与类风湿关节炎（RA）之间的关联已被广泛描述，但 PD 直接参与 RA 的因果关系尚缺乏直接证据。我们研究了口腔（）在 PD 中的启动作用以及随后的 RA，并且评估了大鼠的破骨生物标志物和关节炎发展情况。
Lewis 大鼠经口暴露于 、 或对照凝胶中 1 个月，然后再随访 8 个月。通过血清学、牙龈炎严重程度和微 CT（µCT）评估 PD 的发病和发展。我们使用循环促炎标志物、抗环瓜氨酸肽（CCP）、抗瓜氨酸蛋白抗体（ACPA）、踝关节组织学和 µCT 来研究关节炎的发展。
仅在经口暴露于 的大鼠中观察到 PD，早在暴露后 1 个月即可观察到。仅在 组中观察到关节和全身炎症，在 4 和 8 个月后。8 个月时，在踝关节中观察到炎性细胞浸润，与皮质侵蚀和整体皮质骨减少平行。此外，抗 CCP2 与局部和全身骨丢失相关。
在我们的长期研究中，经口暴露于 引起的 PD 引发了血清阳性关节炎，伴有全身炎症和骨侵蚀。这是口腔启动作用诱导关节炎的首例体内证明。
