Chen Si, Du Chang
Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou Guangdong, 510641, P.R.China;National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou Guangdong, 510006, P.R.China;Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou Guangdong, 510006, P.R.China.
Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou Guangdong, 510641, P.R.China;National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou Guangdong, 510006, P.R.China;Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou Guangdong, 510006,
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Sep 15;32(9):1123-1130. doi: 10.7507/1002-1892.201804127.
To investigate the preparation and osteogenic properties of poly ( -lactic acid)(PLLA)/lecithin porous scaffolds with open pore structure.
PLLA/lecithin porous scaffolds with different lecithin contents (0, 5%, 10%, 20%, 30%, 40%, 50%) were prepared by thermally induced phase separation (groups A, B, C, D, E, F, and G, respectively). Scanning electron microscopy (SEM) was used to observe the surface morphology of the scaffolds. Wide-angle X-ray diffraction (XRD) and differential scanning calorimetry (DSC) were used to detect the crystallinity of the scaffolds. The water uptake ability of the scaffolds was measured. The cell growth and viability of bone marrow mesenchymal stem cells (BMSCs) of mouse on each scaffold was assessed by cell counting kit 8 (CCK-8) method. The osteogenic differentiation ability of BMSCs on each scaffold was evaluated by alkaline phosphatase (ALP) activity. Finally, a critical-size rat calvarial bone defect model was used to evaluate the osteogenesis of the scaffolds . Micro-CT was used to reconstruct the three-dimensional model of the defect area, and the bone volume and bone mineral density were quantitatively analyzed.
SEM results showed that the lecithin could slightly reduce the pore size; when lecithin content was 50%, platelet-like structure could be observed on the scaffolds. Wide angle XRD and DSC showed that the crystallinity of scaffolds gradually decreased with the increase of lecithin content. The water uptake ability test showed that the hydrophilicity of scaffolds increased with the increase of lecithin content. CCK-8 assay showed that cell activity gradually increased with the increase of culture time. After 7 days of culture, the absorbance ( ) value of groups C, D, E, and F were significantly higher than that of groups A, B, and G ( <0.05), but no significant difference was found among groups C, D, E, and F ( >0.05). After 14 days of osteogenic induction, with the increase of lecithin content, there was a significant difference in ALP activity of each group. The ALP activity in groups D, E, F, and G were significantly higher than that in groups A, B, and C ( <0.05). , the results of Micro-CT examination and bone volume and bone mineral density showed that the scaffolds with 30% lecithin had the best repairing effect.
Prepared by thermally induced phase separation, the cytocompatibility, osteogenic differentiation, and bone repair ability of the PLLA/lecithin porous scaffold is obviously better than that of pure PLLA scaffold. PLLA/lecithin porous scaffold with suitable lecithin content is a promising scaffold material for bone tissue engineering.
研究具有开孔结构的聚左旋乳酸（PLLA）/卵磷脂多孔支架的制备及其成骨性能。
采用热致相分离法制备了卵磷脂含量不同（分别为0、5%、10%、20%、30%、40%、50%）的PLLA/卵磷脂多孔支架（分别为A、B、C、D、E、F和G组）。用扫描电子显微镜（SEM）观察支架的表面形貌。采用广角X射线衍射（XRD）和差示扫描量热法（DSC）检测支架的结晶度。测定支架的吸水能力。采用细胞计数试剂盒8（CCK-8）法评估小鼠骨髓间充质干细胞（BMSCs）在各支架上的细胞生长和活力。通过碱性磷酸酶（ALP）活性评估BMSCs在各支架上的成骨分化能力。最后，采用临界尺寸大鼠颅骨骨缺损模型评估支架的成骨作用。用Micro-CT重建缺损区域的三维模型，并对骨体积和骨密度进行定量分析。
SEM结果显示，卵磷脂可使孔径略有减小；当卵磷脂含量为50%时，在支架上可观察到片状结构。广角XRD和DSC显示，支架的结晶度随卵磷脂含量的增加而逐渐降低。吸水能力测试表明，支架的亲水性随卵磷脂含量的增加而增强。CCK-8检测显示，细胞活性随培养时间的增加而逐渐增强。培养7天后，C、D、E和F组的吸光度（ ）值显著高于A、B和G组（ <0.05），但C、D、E和F组之间无显著差异（ >0.05）。成骨诱导14天后，随着卵磷脂含量的增加，各组ALP活性存在显著差异。D、E、F和G组的ALP活性显著高于A、B和C组（ <0.05）。 ，Micro-CT检查结果以及骨体积和骨密度显示，卵磷脂含量为30%的支架修复效果最佳。
通过热致相分离制备的PLLA/卵磷脂多孔支架的细胞相容性、成骨分化能力和骨修复能力明显优于纯PLLA支架。具有合适卵磷脂含量的PLLA/卵磷脂多孔支架是一种有前景的骨组织工程支架材料。
