Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China.
J Mater Sci Mater Med. 2012 Nov;23(11):2639-48. doi: 10.1007/s10856-012-4721-4. Epub 2012 Jul 20.
In this study, natural lecithin was incorporated into cholesterol-poly(ε-caprolactone) (Chol-PCL) by solution blending in order to modify the performance of the hydrophobic and bio-inert PCL. The fibrous Chol-PCL/lecithin membranes were fabricated by electrospinning, and the surface morphology and properties were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, static water contact angle, and mechanical tensile testing. The blood compatibility of the scaffolds was evaluated by in vitro hemolysis assay. The cytocompatibility of the scaffolds was investigated by cell adhesion and proliferation using bone-marrow mesenchymal stem cells (MSCs). Subcutaneous implantation was also performed to evaluate the in vivo inflammatory reaction. The tubular tissue-engineered vascular graft (TEVG) was further constructed by rolling cell sheet comprising fibrous membrane and MSCs. Furthermore, endothelial cells (ECs) were seeded onto the lumen of the graft with the aim to form vascular endothelium. The preliminary results indicate that electrospun Chol-PCL/lecithin scaffolds show improved hemocompatibility and cytocompatibility compared with neat Chol-PCL, and combining the Chol-PCL/lecithin fibrous scaffold with MSCs and ECs with well controlled distribution is a promising strategy for constructing TEVGs.
在这项研究中，通过溶液共混将天然卵磷脂掺入胆固醇-聚（ε-己内酯）（Chol-PCL）中，以改善疏水性和生物惰性 PCL 的性能。通过静电纺丝制备了 Chol-PCL/卵磷脂纤维膜，并通过扫描电子显微镜、X 射线光电子能谱、静态水接触角和力学拉伸试验对其表面形貌和性能进行了表征。通过体外溶血试验评估了支架的血液相容性。通过骨髓间充质干细胞（MSCs）的细胞黏附和增殖来研究支架的细胞相容性。还进行了皮下植入以评估体内炎症反应。进一步通过包含纤维膜和 MSCs 的细胞片滚动构建管状组织工程血管移植物（TEVG）。此外，为了形成血管内皮，将内皮细胞（ECs）接种到移植物的管腔中。初步结果表明，与纯 Chol-PCL 相比，静电纺丝 Chol-PCL/卵磷脂支架具有更好的血液相容性和细胞相容性，将 Chol-PCL/卵磷脂纤维支架与 MSC 和具有良好控制分布的 ECs 结合是构建 TEVG 的一种很有前途的策略。
