Wang Y, Cui F Z, Jiao Y P, Hu K, Fan D D
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Biomed Mater. 2008 Mar;3(1):015012. doi: 10.1088/1748-6041/3/1/015012. Epub 2008 Mar 3.
Lecithin was blended to improve the hydrophilicity and biocompatibility of bone graft containing poly(l-lactic acid) (PLLA). Solution blending and freeze drying were used to fabricate symmetrical scaffolds containing different percentages of lecithin (lecithin: PLLA = 0, 5, 10 wt%). Scanning electron microscopy showed that the scaffolds maintained the three-dimensional porous structure. A water uptake experiment proved the significant improvement of hydrophilicity of the blend scaffold. With the addition of lecithin, the compressive strength and compressive modulus decreased. When the weight ratio of lecithin to PLLA was up to 10%, the compressive strength was still more than the lower limit of natural cancellous bone. To test the biocompatibility of the scaffolds, cell culture in vitro and subcutaneous implantation in vivo were performed. MC3T3-E1 preosteoblastic cells were cultured on the scaffolds for 7 days. Methylthiazol tetrazolium assay and laser scanning confocal microscopy were used to exhibit proliferation and morphology of the cells. The subcutaneous implantation in rats tested inflammatory response to the scaffolds. The results proved the better biocompatibility and milder inflammatory reactions of the blend scaffold (lecithin: PLLA = 5%) compared with the scaffold without lecithin. The modified scaffold containing lecithin is promising for bone tissue engineering.
将卵磷脂进行混合以改善含聚（L-乳酸）（PLLA）骨移植材料的亲水性和生物相容性。采用溶液共混和冷冻干燥法制备了含有不同百分比卵磷脂（卵磷脂：PLLA = 0、5、10 wt%）的对称支架。扫描电子显微镜显示支架保持了三维多孔结构。吸水实验证明了共混支架亲水性的显著提高。随着卵磷脂的添加，抗压强度和抗压模量降低。当卵磷脂与PLLA的重量比高达10%时，抗压强度仍超过天然松质骨的下限。为测试支架的生物相容性，进行了体外细胞培养和体内皮下植入实验。将MC3T3-E1前成骨细胞在支架上培养7天。采用甲基噻唑四氮唑法和激光扫描共聚焦显微镜观察细胞的增殖和形态。大鼠皮下植入实验测试了支架的炎症反应。结果证明，与不含卵磷脂的支架相比，共混支架（卵磷脂：PLLA = 5%）具有更好的生物相容性和更轻微的炎症反应。含卵磷脂的改性支架在骨组织工程方面具有广阔前景。
