Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Nanomedicine (Lond). 2019 Mar;14(6):675-687. doi: 10.2217/nnm-2018-0173. Epub 2019 Jan 31.
To evaluate the antibiofilm effect and esp gene downregulation of Enterococcus faecalis through nanozinc oxide fabricated on natural zeolite (NanoZnO/Ze). Materials & methods:Zeolite and NanoZnO/Ze materials were characterized by x-ray diffraction, x-ray fluorescence and field emission scanning electron microscopy coupled with energy dispersive x-ray. Atomic absorption spectroscopy was used to evaluate zinc release. E. faecalis biofilm formation and its esp gene expression were assessed under nanocomposite treatment.
Spherical-shaped ZnO nanoparticles with an average size of 30 nm were dispersed on the zeolites surface. The leakage of cationic zinc from NanoZnO/Ze displayed a long lasting and considerable release content (p < 0.0001) compared with ZnO/Ze. NanoZnO/Ze effectively inhibited (p < 0.0001) biofilm formation and affected esp gene downregulation of E. faecalis.
Our results show that NanoZnO/Zeolite can potentiate against biofilm infections due to E. faecalis and possibly other pathogens.
评估纳米氧化锌负载天然沸石（NanoZnO/Ze）对粪肠球菌生物膜的抑制作用及其对 esp 基因表达的下调作用。
采用 X 射线衍射、X 射线荧光和场发射扫描电子显微镜结合能谱对沸石和 NanoZnO/Ze 材料进行了表征。原子吸收光谱法用于评估锌的释放情况。在纳米复合材料处理下，评估粪肠球菌生物膜的形成及其 esp 基因的表达。
在沸石表面分散着平均粒径为 30nm 的球形 ZnO 纳米粒子。与 ZnO/Ze 相比，NanoZnO/Ze 中阳离子锌的泄漏表现出持久且相当大的释放含量（p<0.0001）。NanoZnO/Ze 能有效抑制（p<0.0001）粪肠球菌生物膜的形成，并影响其 esp 基因的下调。
我们的结果表明，NanoZnO/Zeolite 可能通过抑制粪肠球菌和其他潜在病原体的生物膜感染而具有潜在的应用价值。
