Garfin D E, Stites D P, Zitnik L A, Prusiner S B
J Immunol. 1978 Jun;120(6):1986-90.
A consistent modification in B lymphocyte activation has been observed 1 month after infection of C3H/HeJ mice with scrapie. The mitogenic response to lipopolysaccharide of splenocyte cultures from experimental mice was reduced 30 to 60% as compared to controls. This reduction in mitogen responsiveness was transient but coincided with the onset of detectable splenomegaly and with the reported recovery of maximum yield of infectious scrapie agent in the spleen. The DNA synthetic response to lipopolysaccharide stimulation of splenocytes from scrapie-infected C3H/HeJ mice was depressed relative to controls only between 20 and 40 days after intracerebral inoculation. At all other times, experimental and control responses were identical. Scrapie-associated decreases in mitogenesis were found whether the spleen cell cultures contained splenocytes from individual mice, splenocytes pooled from several mice, or gradient-purified mononuclear cells. The responses of C3H/HeJ splenocyte cultures to phytohemagglutinin or concanavalin A stimulation were unaffected by scrapie infection.
在用羊瘙痒病感染C3H/HeJ小鼠1个月后，观察到B淋巴细胞激活存在持续改变。与对照组相比，实验小鼠脾细胞培养物对脂多糖的促有丝分裂反应降低了30%至60%。这种有丝分裂原反应性的降低是短暂的，但与可检测到的脾肿大的出现以及脾脏中传染性羊瘙痒病病原体最大产量的恢复报道同时发生。仅在脑内接种后20至40天内，与对照组相比，感染羊瘙痒病的C3H/HeJ小鼠脾细胞对脂多糖刺激的DNA合成反应受到抑制。在所有其他时间，实验和对照反应相同。无论脾细胞培养物包含来自单个小鼠的脾细胞、几只小鼠汇集的脾细胞还是梯度纯化的单核细胞，均发现有与羊瘙痒病相关的有丝分裂生成减少。C3H/HeJ脾细胞培养物对植物血凝素或刀豆球蛋白A刺激的反应不受羊瘙痒病感染的影响。
