Garfin D E, Stites D P, Perlman J D, Cochran S P, Prusiner S B
J Infect Dis. 1978 Sep;138(3):396-400. doi: 10.1093/infdis/138.3.396.
Spleen weights and mitogen responsiveness of splenocyte cultures from scrapie agent-infected and control-inoculated mice were compared over two-month periods following inoculation. Splenocytes from Swiss, C57B1, and BALB/c mice were stimulated with the T (thymus-derived) cell mitogens phytohemagglutinin or concanavalin A, the B (bone marrow-derived) cell mitogen bacterial lipopolysaccharide, or pokeweed mitogen, a stimulator of both T and B cells. Although significant splenomegaly was associated with scrapie infection, we failed to observe any significant differences in the activation of experimental and control cells. Studies with BALB/c mice suggested the possibility, however, that with both phytohemagglutinin and lipopolysaccharide, specific decreases in lymphocyte activation might occur with more optimal culture conditions. The data are consistent with the idea that the scrapie agent stimulates only subtle immunological changes within the host as it destroys the cells of the central nervous system.
对接种了瘙痒病病原体的小鼠和接种了对照物的小鼠，在接种后的两个月内比较了它们脾脏的重量以及脾细胞培养物对丝裂原的反应性。用T（胸腺来源）细胞丝裂原植物血凝素或刀豆球蛋白A、B（骨髓来源）细胞丝裂原细菌脂多糖或美洲商陆丝裂原（一种T和B细胞的刺激物）刺激来自瑞士小鼠、C57B1小鼠和BALB/c小鼠的脾细胞。虽然显著的脾肿大与瘙痒病感染有关，但我们未能观察到实验组和对照组细胞在激活方面有任何显著差异。然而，对BALB/c小鼠的研究表明，在植物血凝素和脂多糖这两种情况下，在更理想的培养条件下可能会出现淋巴细胞激活的特异性降低。这些数据与以下观点一致，即瘙痒病病原体在破坏中枢神经系统细胞时，仅在宿主体内刺激细微的免疫变化。
