聚焦 DNA：单分子方法可视化 DNA。

School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong 2522, Australia.

Illawarra Health and Medical Research Institute, Wollongong, New South Wales 2522, Australia.

Molecules. 2019 Jan 30;24(3):491. doi: 10.3390/molecules24030491.

The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein⁻DNA interactions. Imaging methods using small molecules and protein-based probes to visualise DNA have propelled our understanding of complex biochemical reactions involving DNA. This review focuses on summarising some of the methodological developments made to visualise individual DNA molecules and discusses how these probes have been used in single-molecule biophysical assays.

能够观察单个 DNA 分子已经彻底改变了我们研究与核酸有关的生物转化的方式。已经开发出许多策略来操纵 DNA 分子，以研究机械性质、动力学和蛋白质-DNA 相互作用。使用小分子和基于蛋白质的探针进行成像的方法推动了我们对涉及 DNA 的复杂生化反应的理解。本综述重点总结了一些用于可视化单个 DNA 分子的方法学进展，并讨论了这些探针如何用于单分子生物物理测定。