Morrell E M, Surwit R S, Kuhn C M, Feinglos M N, Cochrane C
University of Michigan, Ann Arbor.
Psychosom Med. 1988 Nov-Dec;50(6):586-90. doi: 10.1097/00006842-198811000-00004.
The obese (C57BL/6J ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. Recent experiments have shown that stress hyperglycemia can be classically conditioned in the obese but not in the lean mouse. In the present study, classical conditioning of insulin secretion was attempted in C57BL/6J obese and lean animals. For 21 days, obese and lean mice were exposed to a conditioned olfactory stimulus prior to and during eating. On the 22nd day, blood was sampled for all animals following presentation of the conditioned stimulus; testing was repeated 2 weeks later following an additional 4 days of conditioning. Results indicated an effect of conditioning, with significantly greater plasma insulin for trained than for untrained obese mice. That insulin secretion can be more easily conditioned in the obese mouse suggests that a cholinergic mechanism may be involved in the hyperinsulinemia characteristic of this animal.
肥胖（C57BL/6J ob/ob）小鼠是常用的非胰岛素依赖型糖尿病动物模型。最近的实验表明，应激性高血糖可在肥胖小鼠中形成经典条件反射，但在瘦小鼠中则不能。在本研究中，尝试在C57BL/6J肥胖和瘦动物中对胰岛素分泌进行经典条件反射。在21天的时间里，肥胖和瘦小鼠在进食前和进食期间暴露于条件性嗅觉刺激。在第22天，在呈现条件刺激后对所有动物进行采血；在额外进行4天条件反射后，2周后重复测试。结果表明存在条件反射效应，训练过的肥胖小鼠的血浆胰岛素水平显著高于未训练的肥胖小鼠。胰岛素分泌在肥胖小鼠中更容易形成条件反射，这表明胆碱能机制可能与该动物的高胰岛素血症特征有关。
