Surwit R S, McCubbin J A, Livingston E G, Feinglos M N
Psychosom Med. 1985 Nov-Dec;47(6):565-8. doi: 10.1097/00006842-198511000-00006.
The obese (C57BL/6J ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. It has recently been demonstrated that this mouse is not consistently hyperglycemic, however, unless it is subjected to environmental stress. In the present study, hyperglycemia in obese mice was induced by classical conditioning. Obese diabetic mice and lean control animals were exposed to shaking stress. All animals developed hyperglycemia in response to shaking. To demonstrate classical conditioning, some obese and lean animals were exposed to a metronome prior to and during the shaking. Other animals were exposed to the metronome and shaking in a noncontingent fashion and one group of animals was exposed to the metronome without any exposure to shaking. All animals received seven exposures to one of the three above conditions over a 3-day period. On the 4th day all animals were exposed to the metronome alone, following which blood samples were drawn. Classical conditioning of stress hyperglycemia was demonstrated in obese, but not in lean, mice.
肥胖（C57BL/6J ob/ob）小鼠是一种常用的非胰岛素依赖型糖尿病动物模型。然而，最近有研究表明，这种小鼠并非始终处于高血糖状态，除非它受到环境压力。在本研究中，通过经典条件反射诱导肥胖小鼠出现高血糖。将肥胖糖尿病小鼠和瘦型对照动物暴露于摇晃应激中。所有动物在受到摇晃时都会出现高血糖。为了证明经典条件反射，一些肥胖和瘦型动物在摇晃之前和期间接触节拍器。其他动物以非偶然的方式接触节拍器和摇晃，还有一组动物只接触节拍器而不接触摇晃。在3天的时间内，所有动物都接受了上述三种条件之一的七次暴露。在第4天，所有动物仅暴露于节拍器，之后采集血样。结果表明，肥胖小鼠出现了应激性高血糖的经典条件反射，而瘦型小鼠则没有。
