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一种用于检测IgA、IgG和IgM类风湿因子（RF）的酶联免疫吸附测定法。一、测定参数。

An ELISA for IgA, IgG and IgM-RF measurement. I. Parameters of the assay.

作者信息

Ruschen S, Lemm G, Warnatz H

机构信息

Dept. Rheumatology and Clinical Immunology, Katholisches Krankenhaus Essen-Werden.

出版信息

Scand J Rheumatol Suppl. 1988;75:32-5. doi: 10.3109/03009748809096736.

DOI:10.3109/03009748809096736
PMID:3070729
Abstract

A simple and rapid solid phase ELISA for the stepless determination of IgA, IgG and IgM-RF was developed. The ELISA works with Fc parts of human IgG as antigen. Specificity, validity and precision were tested. RF complex dissociation by urea and separation by gel filtration was performed in several samples to obtain information of the transfer of non-RF-IgG by pentameric IgM-RF. The RF-ELISA may give better information on the significance of RF of the three Ig classes in the clinical course of RA.

摘要

开发了一种用于无分级测定IgA、IgG和IgM-RF的简单快速固相酶联免疫吸附测定法（ELISA）。该ELISA以人IgG的Fc部分作为抗原发挥作用。对特异性、有效性和精密度进行了测试。在几个样品中进行了尿素介导的RF复合物解离和凝胶过滤分离，以获取五聚体IgM-RF转移非RF-IgG的相关信息。RF-ELISA可能会为类风湿性关节炎（RA）临床病程中三种Ig类别的RF的意义提供更好的信息。

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