Grunnet N, Espersen G T
Department of Clinical Immunology, Aalborg Hospital, Denmark.
Scand J Rheumatol Suppl. 1988;75:36-9. doi: 10.3109/03009748809096737.
In the literature it is seen that the antigens used in Elisa test for rheumatoid factors (RF) are either human IgG, rabbit IgG, or mouse IgG. We investigated RF-IgA activity in 55 serum samples using either human IgG or rabbit IgG as antigen. In other 76 serum samples we investigated RF-IgM activity using either human IgG or rabbit IgG as antigen. In RF-IgA the median AU/ml is found a factor 2-3 times higher using rabbit IgG as antigen. The discriminatory power of the RF-IgA Elisa test is better using rabbit IgG as antigen. In RF-IgM Elisa the results are different using human or rabbit IgG as antigen. Our investigation illustrates some of the problems in Elisa testing for RF's. We conclude that the variations are influenced by the antigen source, being rabbit or human IgG. This might affect the daily and scientific communication between different laboratories and/or clinical departments. Results obtained by one antigen cannot easily be compared with results obtained with the other antigen. This means that the user of the results has to be aware of the antigen used, and it seems most important to develop some kind of standardization in the Elisa tests used.
在文献中可以看到，用于类风湿因子（RF）酶联免疫吸附测定（ELISA）的抗原要么是人IgG、兔IgG，要么是小鼠IgG。我们使用人IgG或兔IgG作为抗原，对55份血清样本中的RF-IgA活性进行了研究。在另外76份血清样本中，我们使用人IgG或兔IgG作为抗原，对RF-IgM活性进行了研究。在RF-IgA检测中，以兔IgG作为抗原时，发现中位数AU/ml要高出2至3倍。以兔IgG作为抗原时，RF-IgA ELISA检测的鉴别能力更强。在RF-IgM ELISA检测中，用人IgG或兔IgG作为抗原时结果有所不同。我们的研究揭示了RF ELISA检测中的一些问题。我们得出结论，这些差异受抗原来源（兔IgG或人IgG）的影响。这可能会影响不同实验室和/或临床科室之间的日常交流和学术沟通。用一种抗原获得的结果不易与用另一种抗原获得的结果进行比较。这意味着结果的使用者必须了解所使用的抗原，并且在所用的ELISA检测中制定某种标准化似乎最为重要。
