Department of Biology, University of Naples "Federico II", Naples, Italy.
Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Naples, Italy.
Chemosphere. 2019 May;222:243-249. doi: 10.1016/j.chemosphere.2019.01.140. Epub 2019 Jan 24.
Aluminium and cadmium are biologically non-essential metals with a role in neurodegenerative and neuromuscular diseases. As an attractive model for neurobehavioural studies, zebrafish at 6 h post fertilization were exposed to 9, 18, 36 and 72 μM CdCl and 50, 100 and 200 μM AlCl, respectively, for 72 h, and motility such as distance moved, mean velocity, cumulative movement, meander and heading were measured by DanioVision equipment. The hatching time was also analysed. A delay in the exit from the chorion was observed in all treated larvae with respect to the controls. CdCl acted on the exit from the chorion of larvae with a dose-dependent delay. By contrast, the delay caused by AlCl was greater at low concentrations. A dose-dependent reduction in swimming performance was observed in the larvae exposed to CdCl. Instead, for those exposed to AlCl, swimming performance improved at higher concentrations although values were in general lower than those of control. All the parameters had a similar trend except the meander parameter which showed a dose-dependent reduction. These data show that cadmium and aluminium can delay hatching and alter swimming ability in the early developmental stages of zebrafish, albeit with different effects, suggesting that exposure to sublethal concentrations of both metals can change behavioural parameters.
铝和镉是生物上非必需的金属，在神经退行性和神经肌肉疾病中发挥作用。斑马鱼在受精后 6 小时作为神经行为研究的有吸引力的模型，分别暴露于 9、18、36 和 72 μM CdCl 和 50、100 和 200 μM AlCl 中 72 小时，通过 DanioVision 设备测量运动，如移动距离、平均速度、累计运动、曲折和航向。还分析了孵化时间。与对照组相比，所有处理过的幼虫的卵壳出口都出现了延迟。CdCl 对幼虫的卵壳出口有剂量依赖性延迟作用。相比之下，AlCl 在低浓度时引起的延迟更大。暴露于 CdCl 的幼虫的游泳性能呈剂量依赖性降低。相反，暴露于 AlCl 的幼虫在较高浓度时游泳性能提高，尽管总体上低于对照组。除了曲折参数表现出剂量依赖性降低外，所有参数都有相似的趋势。这些数据表明，镉和铝可以延迟斑马鱼早期发育阶段的孵化，并改变游泳能力，尽管效果不同，这表明暴露于亚致死浓度的两种金属都会改变行为参数。
