Ruales-Salcedo Angela Viviana, Higuita Juan Carlos, Fontalvo Javier, Woodley John M
Grupo de Investigación en Aplicación de Nuevas Tecnologías, Departamento de Ingeniería Química, Universidad Nacional de Colombia, Campus La Nubia, Edificio L103, Manizales, Colombia.
Grupo de Procesos Químicos, Catalíticos y Biotecnológicos, Departamento de Ingeniería Química, Universidad Nacional de Colombia, Campus La Nubia, Edificio L103, Manizales, Colombia.
Z Naturforsch C J Biosci. 2019 Feb 25;74(3-4):77-84. doi: 10.1515/znc-2018-0190.
While the application of enzymes to synthetic and industrial problems continues to grow, the major development today is focused on multi-enzymatic cascades. Such systems are particularly attractive, because many commercially available enzymes operate under relatively similar operating conditions. This opens the possibility of one-pot operation with multiple enzymes in a single reactor. In this paper the concept of modules is introduced whereby groups of enzymes are combined in modules, each operating in a single reactor, but with the option of various operating strategies to avoid any complications of nonproductive interactions between the enzymes, substrates or products in a given reactor. In this paper the selection of modules is illustrated using the synthesis of the bulk chemical, gluconic acid, from lignocellulosic waste.
虽然酶在合成和工业问题中的应用不断增加，但当今的主要发展集中在多酶级联反应上。这样的系统特别有吸引力，因为许多市售酶在相对相似的操作条件下运行。这为在单个反应器中使用多种酶进行一锅法操作提供了可能性。本文引入了模块的概念，即将酶组合成模块，每个模块在单个反应器中运行，但可以选择各种操作策略，以避免给定反应器中酶、底物或产物之间非生产性相互作用带来的任何复杂性。本文以从木质纤维素废料合成大宗化学品葡萄糖酸为例，说明了模块的选择。
