Ren Jialei, Li Piwu, Wei Xiaofeng, Wang Jianbin, Guo Chuanzhuang, Liu Keyi, Wang Junqing, Li Xia
State Key Laboratory of Green Papermaking and Resource Recycling, Shandong Academy of Science, Qilu University of Technology, Jinan, China.
School of Bioengineering, Shandong Academy of Science, Qilu University of Technology, Jinan, China.
Front Bioeng Biotechnol. 2025 Aug 18;13:1607782. doi: 10.3389/fbioe.2025.1607782. eCollection 2025.
Sodium gluconate has a wide range of applications, including in the fields of construction, textiles, medicine, the chemical industry, and food, so the industrialized production of sodium gluconate is particularly important. However, the preparation process of sodium gluconate is not mature enough, and the production cost is high, which restricts the development of the industry. In this study, the optimization of process conditions for the catalytic production of sodium gluconate from glucose via a dual-enzyme system of glucose oxidase (GOD) and catalase (CAT) was investigated in detail. Factors such as pH, temperature, metal ions, enzyme addition, stirring speed, and aeration were examined. After optimizing these parameters through one-way experiments, the Box-Behnken design (BBD) was employed to refine the process further, focusing on stirring speed, enzyme addition, and aeration. The optimal reaction conditions were identified as follows: a reaction pH of 5.9, a reaction temperature of 38°C, enzyme addition of 0.2%, batch addition, 80% GOD at 0 h, 20% GOD at 2 h, stirring speed of 700 rpm, aeration amount of 1.2 vvm, and a tank pressure of 0.04 Pa. Under these conditions, the reaction cycle for sodium gluconate production was reduced to 7.75 ± 0.5 h. These optimized conditions significantly improve existing methods, offering a more efficient and cost-effective approach to sodium gluconate production. The findings provide valuable insights for scaling up biocatalytic processes, with the potential for a substantial industrial impact, particularly in reducing production costs and improving sustainability in the chemical and food industries.
葡萄糖酸钠具有广泛的应用，包括在建筑、纺织、医药、化工和食品等领域，因此葡萄糖酸钠的工业化生产尤为重要。然而，葡萄糖酸钠的制备工艺尚不够成熟，生产成本较高，这限制了该行业的发展。在本研究中，详细研究了通过葡萄糖氧化酶（GOD）和过氧化氢酶（CAT）的双酶系统催化葡萄糖生产葡萄糖酸钠的工艺条件优化。考察了pH、温度、金属离子、酶添加量、搅拌速度和通气量等因素。通过单因素实验对这些参数进行优化后，采用Box-Behnken设计（BBD）进一步优化工艺，重点关注搅拌速度、酶添加量和通气量。确定的最佳反应条件如下：反应pH为5.9，反应温度为38°C，酶添加量为0.2%，分批添加，0 h时添加80%的GOD，2 h时添加20%的GOD，搅拌速度为700 rpm，通气量为1.2 vvm，罐压为0.04 Pa。在这些条件下，葡萄糖酸钠生产的反应周期缩短至7.75±0.5 h。这些优化条件显著改进了现有方法，为葡萄糖酸钠生产提供了一种更高效、更具成本效益的方法。研究结果为扩大生物催化工艺规模提供了有价值的见解，具有巨大的工业影响潜力，特别是在降低生产成本和提高化工及食品行业的可持续性方面。
