Richens E R, Tungekar F M, Behbehani K
Department of Microbiology, Faculty of Medicine, University of Kuwait.
Acta Diabetol Lat. 1988 Jul-Sep;25(3):185-95. doi: 10.1007/BF02624813.
Using islets obtained by collagenase digestion, we have carried out a longitudinal study of the ultrastructure of endocrine cells of the islets of Langerhans from C57BL/6 mice, during the development of diabetes induced by the multiple low-dose streptozotocin (mld STZ) technique, with and without co-treatment with complete Freund's adjuvant (CFA). Diabetes, as assessed by glycemia, occurred on different days in the various treatment groups following the initial STZ injection. It was observed on day 7 in 4/7 and 3/7 animals treated with mld STZ and with CFA plus mld STZ respectively, on day 14 in 3/6 animals treated with CFA plus sub mld STZ and on day 21 in 2/5 animals treated with sub mld STZ. Breakages in the basement membrane of the B-cell, and severe vacuolation, occurred in all STZ treated animals, despite normoglycemia in animals in the sub-mld group. Mononuclear cells (macrophages acutely and lymphocytes chronically) were observed in islets from animals with mld STZ irrespective of treatment with CFA, but were found adjacent to acinar cells only in those animals treated with CFA. Damage to acinar cells was observed exclusively in this group. In sub mld STZ treated animals, mononuclear cells were found only in those animals treated with CFA. These findings are discussed with reference to the etiopathology of the disease.
利用胶原酶消化获得的胰岛，我们对C57BL/6小鼠胰岛内分泌细胞的超微结构进行了纵向研究，研究在采用多次低剂量链脲佐菌素（mld STZ）技术诱导糖尿病的过程中，有无联合使用完全弗氏佐剂（CFA）的情况。通过血糖评估，糖尿病在初次注射STZ后的不同天数出现在各个治疗组的动物身上。分别在第7天出现在4/7只接受mld STZ治疗和3/7只接受CFA加mld STZ治疗的动物中，在第14天出现在3/6只接受CFA加亚mld STZ治疗的动物中，在第21天出现在2/5只接受亚mld STZ治疗的动物中。尽管亚mld组动物血糖正常，但所有接受STZ治疗的动物的B细胞基底膜均出现断裂，且有严重空泡化现象。在接受mld STZ治疗的动物的胰岛中观察到单核细胞（急性为巨噬细胞，慢性为淋巴细胞），无论是否接受CFA治疗，但仅在接受CFA治疗的动物中发现单核细胞邻近腺泡细胞。仅在该组中观察到腺泡细胞受损。在接受亚mld STZ治疗的动物中，仅在接受CFA治疗的动物中发现单核细胞。结合该疾病的病因病理学对这些发现进行了讨论。
