Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad No. 940, Aguascalientes, AGS, Mexico.
Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, 34120, Durango, DGO, Mexico.
Microb Pathog. 2019 Apr;129:56-63. doi: 10.1016/j.micpath.2019.01.041. Epub 2019 Jan 30.
Histopathological studies of human sporotrichosis lesions show pyogenic and granulomatous processes in which polymorphonuclear neutrophils (PMNs) play a central role. Few studies regarding the events associated with the interaction of human PMNs with Sporothrix schenckii have been made despite their importance in the clinical manifestations of the disease. In this study, human PMNs were co-cultured with conidia or yeast cells of S. schenckii to compare the phagocytic activity and morphological changes that could provide a clearer insight into the role of these phagocytes in the initial phase of sporotrichosis. PMNs showed increased cell size and separation of the nuclear lobes after phagocytosis. Through Scanning Electron Microscopy (SEM) analysis, an increase in cells with flattened filaments and vesicles on their surface was observed. Phagocytosed conidia showed a significant increase in width and size. The phagocytic activity was greater against yeasts than with conidia, but the viability of both S. schenckii cellular morphotypes was not drastically affected even after 2 h of co-culture. In conclusion, morphological changes in PMNs suggest that S. schenckii induces processes that may favor proinflammatory events. These phagocytes show a high ability to bind or ingest S. schenckii cells without affecting their viability. Morphological changes recorded in ingested conidia, suggest that this fungus could make the dimorphic switching in PMNs.
人类孢子丝菌病病变的组织病理学研究显示出化脓性和肉芽肿性过程，其中多形核粒细胞（PMN）起着核心作用。尽管多形核粒细胞在疾病的临床表现中很重要，但关于人类 PMN 与申克孢子丝菌相互作用相关事件的研究很少。在这项研究中，将人类 PMN 与申克孢子丝菌的分生孢子或酵母细胞共培养，以比较吞噬活性和形态变化，从而更清楚地了解这些吞噬细胞在孢子丝菌病的初始阶段中的作用。PMN 在吞噬后细胞体积增大，核叶分离。通过扫描电子显微镜（SEM）分析，观察到表面有扁平丝和小泡的细胞数量增加。被吞噬的分生孢子的宽度和大小显著增加。与分生孢子相比，PMN 对酵母的吞噬活性更强，但即使在共培养 2 小时后，两种 S. schenckii 细胞形态的活力也没有受到明显影响。总之，PMN 的形态变化表明申克孢子丝菌诱导了可能有利于促炎事件的过程。这些吞噬细胞具有很高的结合或摄取 S. schenckii 细胞的能力，而不会影响其活力。记录到的被吞噬的分生孢子的形态变化表明，这种真菌可以使 PMN 发生二态性转换。
