Hiruma M, Yamaji K, Shimizu T, Ohata H, Kukita A
Department of Dermatology, National Defense Medical College, Saitama, Japan.
Arch Dermatol Res. 1988;280 Suppl:S94-100.
In the present study, in order to clarify the defense mechanisms against S. schenckii infections, we examined the tissue reactions of mice against the pathogen over a period of time by both light and electron microscopy. The histological features were, at an early stage, a mixed cell granuloma consisting of polymorphonuclear leukocytes (PMNs) and macrophages, and, later on, enlargement and vacuolation of the macrophages at the periphery. In electron microscopy, blastospores had been phagocytized by the PMNs and macrophages, and no extracellular blastospores were seen. PMNs taking up blastospores were phagocytized by other PMNs or by macrophages. After 3 or 4 months, the phagosomes of the macrophages had grown in size, and contained a number of blastospores. During the experiment, the ultrastructure of the blastospores was well preserved, and their viability was considered high. Of the defense mechanisms against S. schenckii infection, PMN phagocytosis is of great importance, but the fact that proliferation of this organism was observed within the macrophages, suggested that the macrophages were not able to destroy the organisms, but rather were responsible for the disease becoming chronic.
在本研究中，为了阐明针对申克孢子丝菌感染的防御机制，我们通过光学显微镜和电子显微镜在一段时间内检查了小鼠对该病原体的组织反应。组织学特征在早期是由多形核白细胞（PMN）和巨噬细胞组成的混合细胞肉芽肿，后期巨噬细胞在外围增大并出现空泡化。在电子显微镜下，芽生孢子已被PMN和巨噬细胞吞噬，未观察到细胞外芽生孢子。吞噬芽生孢子的PMN被其他PMN或巨噬细胞吞噬。3或4个月后，巨噬细胞的吞噬体体积增大，并含有许多芽生孢子。在实验过程中，芽生孢子的超微结构保存良好，其活力被认为较高。在针对申克孢子丝菌感染的防御机制中，PMN吞噬作用非常重要，但在巨噬细胞内观察到该生物体增殖这一事实表明，巨噬细胞无法破坏这些生物体，反而导致疾病慢性化。
