Hiruma M, Kagawa S
Mycopathologia. 1986 Aug;95(2):93-100. doi: 10.1007/BF00437167.
The effects of hyperthermia on phagocytosis and killing of Sporothrix schenckii by polymorphonuclear leukocytes (PMNs) were investigated in order to clarify the mechanism of local thermotherapy for sporotrichosis. Yeast cells of S. schenckii, PMNs and serum were incubated at 37 degrees C or 40 degrees C for 2 or 4 hours. Rate of phagocytosis and killing rate (rate of germination) were estimated, and their processes were observed by transmission electron microscopy. There was no effect of hyperthermia on the phagocytosis rate, but the killing rate increased significantly at 40 degrees C. Electron microscopic examination showed an increase of granularity in the yeast cytoplasm, elongation and fragmentation of the cell membrane. The ultrastructural changes were basically identical under both temperatures, but the degree of these changes was higher at 40 degrees C than at 37 degrees C. Although both intact and degenerated yeasts were found in the same conditions, their transient forms were few, suggesting that the PMN-killing process was completed promptly.
为阐明局部热疗治疗孢子丝菌病的机制，研究了热疗对多形核白细胞（PMN）吞噬和杀灭申克孢子丝菌的影响。将申克孢子丝菌的酵母细胞、PMN和血清在37℃或40℃孵育2或4小时。评估吞噬率和杀灭率（发芽率），并通过透射电子显微镜观察其过程。热疗对吞噬率没有影响，但在40℃时杀灭率显著增加。电子显微镜检查显示酵母细胞质的颗粒度增加，细胞膜伸长和断裂。两种温度下的超微结构变化基本相同，但这些变化的程度在40℃时高于37℃。尽管在相同条件下发现了完整和退化的酵母，但它们的过渡形式很少，这表明PMN杀灭过程迅速完成。
