Department of Obstetrics and Gynecology, University of São Paulo Medical School, São Paulo, Brazil,
Medical Investigation Laboratory (LIM 51), University of São Paulo Medical School, São Paulo, Brazil.
Gynecol Obstet Invest. 2019;84(5):435-444. doi: 10.1159/000496607. Epub 2019 Feb 1.
The objective of this study was to evaluate cytokines related to natural killer and T-regulatory cells in endometriotic lesions, peritoneal fluid (PF) and the peripheral blood (PB) of patients with deep infiltrative endometriosis.
A case-control study was conducted in a tertiary referral hospital. Sixty-four consecutive patients after laparoscopy were divided into 2 groups: with endometriosis (Group A - n = 32) and without endometriosis (Group B - n = 32).
Interleukin (IL)-2, IL-4, IL-7, IL-10, IL-12, IL-15, transforming growth factor β1, and IFNγ concentration was measured using a LuminexTM multiplex suspension bead array. Tissues from endometriotic lesions of patients with endometriosis and from eutopic endometrium were evaluated, as well as PF and PB of all patients.
Compared to the other analyzed groups, IL-15 concentration was significantly higher in the ectopic endometrium and IL-7 in the eutopic endometrium of the endometriosis group (p < 0.05). Compared to endometriosis group, IFNγ, IL-7, and IL-15 were observed to be significantly higher in the PF of the control group, and IL-10 was lower in the control group (p < 0.05). In PB, compared to endometriosis group, IL-4, IL-10, IL-12, IL-15, and IFNγ concentrations were significantly higher in the control group (p < 0.05).
Our hypothesis is that deep endometriosis is a disease out of control. This disease's nature is of progression and invasion of adjacent structures, and proof of this disease state is the disorganized secretion of cytokine regulation and inflammation, which seem to be among the factors responsible for the maintenance of the disease.
本研究旨在评估与自然杀伤和调节性 T 细胞相关的细胞因子在子宫内膜异位症病灶、腹腔液（PF）和深浸润性子宫内膜异位症患者外周血（PB）中的表达。
这是一项在三级转诊医院进行的病例对照研究。64 例腹腔镜检查后的患者被分为 2 组：子宫内膜异位症组（A 组，n=32）和非子宫内膜异位症组（B 组，n=32）。
采用 LuminexTM 多重悬浮珠阵列法测量白细胞介素（IL）-2、IL-4、IL-7、IL-10、IL-12、IL-15、转化生长因子β1 和 IFNγ 的浓度。评估了子宫内膜异位症患者的异位内膜和在位内膜的组织，以及所有患者的 PF 和 PB。
与其他分析组相比，子宫内膜异位症组的异位内膜中 IL-15 浓度显著升高，在位内膜中 IL-7 浓度显著升高（p<0.05）。与子宫内膜异位症组相比，对照组 PF 中的 IFNγ、IL-7 和 IL-15 显著升高，对照组中 IL-10 水平降低（p<0.05）。在 PB 中，与子宫内膜异位症组相比，对照组中 IL-4、IL-10、IL-12、IL-15 和 IFNγ 浓度显著升高（p<0.05）。
我们的假设是深部子宫内膜异位症是一种失控的疾病。这种疾病的本质是进展和侵袭相邻结构，这种疾病状态的证据是细胞因子调节和炎症的失调分泌，这似乎是维持疾病的因素之一。
