Deng Yiqin, Cheng Changhong, Xie Jiawei, Liu Songlin, Ma Hongling, Feng Juan, Su Youlu, Guo Zhixun
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 309 of Building Keyan, 231 Xingang Xi Road, Guangzhou, China.
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
AMB Express. 2019 Feb 2;9(1):18. doi: 10.1186/s13568-019-0745-1.
Increasing evidence has revealed a close association between intestinal bacterial community and hosts health. However, it is unclear whether and what extend Baimang disease alters the intestinal microbiota in mud crab (Scylla paramamosain). Here, we conducted intestinal contents Illumina sequencing of healthy and Baimang diseased mud crab (S. paramamosain) to understand bacterial community variations among health status. In addition, bacterial functional predication was used to investigate whether and how the bacteria variations further change their functions? The phyla of Proteobacteria, Fusobacteria, Cyanobacteria, Tenericutes, Firmicutes, Bacteroidetes, and Spirochaetae constituted over 96.44% of the total intestinal bacteria, with being the dominant taxa. The 7 most significantly different orders, including the increased four orders of Clostridiales, Entomoplasmatales, Bacteroidales, and Mycoplasmatales and the decreased three orders of Vibrionales, Campylobacterales, and Fusobacteriales, accounted for 61.14% dissimilarity, probably being the indicator taxa of Baimang disease. Accordingly, 12 Kyoto Encyclopedia of Genes and Genomes orthologies in level 3 shifted significantly at the diseased crabs. Especially, bacterial secretion system, secretion system, lipopolysaccharide biosynthesis proteins and Vibrio cholerae pathogenic cycle, being related to bacterial virulence, were reduced. In addition, the reduced butanoate metabolism, and induced methane metabolism and one carbon pool by folate were important metabolic processes of probiotic, such as Bacteroides spp. and Clostridium spp., with playing critical roles in host health. This study suggests that Baimang disease coupled altered the intestinal bacterial communities and functions, providing timely information for further analysis the influencing mechanism of Baimang disease in mud crab (S. paramamosain).
越来越多的证据表明肠道细菌群落与宿主健康之间存在密切关联。然而，尚不清楚白芒病是否以及在何种程度上改变了青蟹（拟穴青蟹）的肠道微生物群。在此，我们对健康和患白芒病的青蟹（拟穴青蟹）进行了肠道内容物的Illumina测序，以了解不同健康状态下的细菌群落变化。此外，利用细菌功能预测来研究细菌变化是否以及如何进一步改变其功能？变形菌门、梭杆菌门、蓝细菌门、柔膜菌门、厚壁菌门、拟杆菌门和螺旋体门占肠道细菌总数的96.44%以上，是主要的分类群。7个差异最显著的目，包括增加的梭菌目、昆虫支原体目、拟杆菌目和支原体目以及减少的弧菌目、弯曲菌目和梭杆菌目，占差异的61.14%，可能是白芒病的指示分类群。相应地，3级京都基因与基因组百科全书直系同源物在患病蟹中显著变化。特别是，与细菌毒力相关的细菌分泌系统、分泌系统、脂多糖生物合成蛋白和霍乱弧菌致病循环减少。此外，丁酸代谢减少，甲烷代谢和叶酸诱导的一碳池增加，是拟杆菌属和梭菌属等益生菌的重要代谢过程，对宿主健康起着关键作用。本研究表明，白芒病会改变肠道细菌群落和功能，为进一步分析白芒病对青蟹（拟穴青蟹）的影响机制提供了及时的信息。
