Komura Miyuki, Suzuki Mayuko, Sangsriratanakul Natthanan, Ito Mariko, Takahashi Satoru, Alam Md Shahin, Ono Mizuki, Daio Chisato, Shoham Dany, Takehara Kazuaki
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fchu-shi, Tokyo 183-8509, Japan.
Bar-Ilan University, Begin-Sadat Center for Strategic Studies, Ramat Gan 5290002, Israel.
J Vet Med Sci. 2019 Mar 30;81(3):466-472. doi: 10.1292/jvms.18-0754. Epub 2019 Feb 4.
The inhibitory activities of grapefruit seed extract (GSE) on avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), Salmonella Infantis (SI) and Escherichia coli (EC) were evaluated. Original GSE contained 0.24% benzalkonium chloride (BZC), however, 0.0025% BZC solution could not inactivate bacteria. The activity of diluted GSE (×100, ×500 and ×1,000 with redistilled water) against selected viruses and bacteria was evaluated in this study. The GSE solutions were incubated with the pathogens over a period of time after which the remaining viruses were titrated and the bacterial colonies were counted. In the presence of organic material-5% fetal bovine serum (FBS), the test solutions were sprayed at 1 cm and 30 cm distances to test the efficacy of GSE in a spray form. Furthermore, the efficacy of GSE against bacteria on clothes was tested using non-woven cloth. GSE×100 reduced the viral titer of both AIV and NDV even in 5% FBS condition. IBDV showed high resistance to GSE. GSE×1,000 inactivated both SI and EC within 5 sec, even in the presence of 5% FBS. The disinfectant was able to maintain its efficacy in the spray form at 30 cm distance. GSE was also effective against SI and EC inoculated on fabric. GSE is a potential novel disinfectant against viruses and bacteria, effective even within a short contact time.
评估了葡萄柚籽提取物（GSE）对禽流感病毒（AIV）、新城疫病毒（NDV）、传染性法氏囊病病毒（IBDV）、婴儿沙门氏菌（SI）和大肠杆菌（EC）的抑制活性。原始GSE含有0.24%的苯扎氯铵（BZC），然而，0.0025%的BZC溶液无法灭活细菌。本研究评估了稀释后的GSE（用重蒸馏水稀释100倍、500倍和1000倍）对选定病毒和细菌的活性。将GSE溶液与病原体孵育一段时间后，对剩余病毒进行滴定并对细菌菌落进行计数。在存在有机物质——5%胎牛血清（FBS）的情况下，将测试溶液以1厘米和30厘米的距离进行喷雾，以测试喷雾形式的GSE的功效。此外，使用无纺布测试了GSE对衣物上细菌的功效。即使在5%FBS条件下，GSE×100也降低了AIV和NDV的病毒滴度。IBDV对GSE表现出高抗性。即使在存在5%FBS的情况下，GSE×1000在5秒内就能灭活SI和EC。该消毒剂在30厘米距离的喷雾形式下能够保持其功效。GSE对接种在织物上的SI和EC也有效。GSE是一种潜在的新型病毒和细菌消毒剂，即使在短接触时间内也有效。
