Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
J Vet Med Sci. 2023 Oct 17;85(10):1040-1046. doi: 10.1292/jvms.23-0194. Epub 2023 Aug 29.
The antiviral activity of chlorine dioxide (ClO) in liquid (ClO gas dissolved liquid) and gaseous state against avian influenza virus (AIV) and infectious bronchitis virus (IBV) was evaluated. To evaluate the effect of ClO in liquid state, suspension tests (10 ppm) and carrier tests in dropping / wiping techniques (100 ppm) were performed. In the suspension test, virus titers were reduced below the detection limit within 15 sec after treatment, in spite of the presence of an accompanying organic matter. In the carrier test by dropping technique, AIV and IBV were reduced to below the detection limit in 1 and 3 min, respectively. Following wiping technique, no virus was detected in the wiping sheets after 30 sec of reaction. Both viruses adhering to the carriers were also reduced by 3 logs, thereby indicating that they were effectively inactivated. In addition, the effect of ClO gas against IBV in aerosols was evaluated. After the exposure of sprayed IBV to ClO gas for a few seconds, 94.2% reduction of the virus titer was observed, as compared to the pre-treatment control. Altogether, hence, ClO has an evident potential to be an effective disinfectant for the prevention and control of AIV and IBV infections on poultry farms.
评估了二氧化氯（ClO）在液态（ClO 气体溶解液）和气态下对禽流感病毒（AIV）和传染性支气管炎病毒（IBV）的抗病毒活性。为了评估液态 ClO 的效果，进行了悬浮试验（10 ppm）和滴降/擦拭技术的载体试验（100 ppm）。在悬浮试验中，尽管存在伴随的有机物，处理后 15 秒内病毒滴度降至检测限以下。在滴降技术的载体试验中，AIV 和 IBV 分别在 1 和 3 分钟内减少至检测限以下。擦拭技术 30 秒后，反应后的擦拭纸上未检测到病毒。附着在载体上的两种病毒也减少了 3 个对数，表明它们被有效灭活。此外，还评估了 ClO 气体对气溶胶中 IBV 的作用。将喷雾 IBV 暴露于 ClO 气体几秒钟后，与预处理对照相比，病毒滴度降低了 94.2%。总之，ClO 具有成为农场中预防和控制 AIV 和 IBV 感染的有效消毒剂的明显潜力。
