Toyofuku Chiharu, Alam Md Shahin, Yamada Masashi, Komura Miyuki, Suzuki Mayuko, Hakim Hakimullah, Sangsriratanakul Natthanan, Shoham Dany, Takehara Kazuaki
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Bar-Ilan University, Begin-Sadat Center for Strategic Studies, Ramat Gan 5290002, Israel.
J Vet Med Sci. 2017 Jun 16;79(6):1019-1023. doi: 10.1292/jvms.17-0089. Epub 2017 May 12.
An alkaline agent, namely food additive grade calcium hydroxide (FdCa(OH)) in solution at 0.17%, was evaluated for its bactericidal efficacies in chiller water with sodium hypochlorite (NaOCl) at a concentration of 200 ppm total residual chlorine. Without organic material presence, NaOCl could inactivate Salmonella Infantis and Escherichia coli within 5 sec, but in the presence of fetal bovine serum (FBS) at 0.5%, the bactericidal effects of NaOCl were diminished completely. FdCa(OH) solution required 3 min to inactivate bacteria with or without 5% FBS. When NaOCl and FdCa(OH) were mixed at the final concentration of 200 ppm and 0.17%, respectively, the mixed solution could inactivate bacteria at acceptable level (10 reduction of bacterial titer) within 30 sec in the presence of 0.5% FBS. The mixed solution also inhibited cross-contamination with S. Infantis or E. coli on chicken meats. It was confirmed and elucidated that FdCa(OH) has a synergistic effect together with NaOCl for inactivating microorganisms.
评估了一种碱性试剂，即食品添加剂级氢氧化钙（FdCa(OH)），其在溶液中的浓度为0.17%，研究了它在总余氯浓度为200 ppm的次氯酸钠（NaOCl）冷却水中的杀菌效果。在没有有机物质存在的情况下，NaOCl可在5秒内使婴儿沙门氏菌和大肠杆菌失活，但在存在0.5%胎牛血清（FBS）的情况下，NaOCl的杀菌效果完全减弱。FdCa(OH)溶液无论有无5% FBS，都需要3分钟才能使细菌失活。当NaOCl和FdCa(OH)分别以200 ppm和0.17%的终浓度混合时，在存在0.5% FBS的情况下，混合溶液可在30秒内将细菌灭活至可接受水平（细菌滴度降低10倍）。该混合溶液还可抑制鸡肉上婴儿沙门氏菌或大肠杆菌的交叉污染。已证实并阐明，FdCa(OH)与NaOCl一起对灭活微生物具有协同作用。
