Ghaziasgar F S, Poursina F, Hassanzadeh A
MSc student of Medical microbiology, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
Ann Ig. 2019 Mar-Apr;31(2):154-164. doi: 10.7416/ai.2019.2268.
The aim of this study was to determine and compare antibiotic resistance profile, biofilm formation ability and frequency of agg and ace genes in Enterococcus spp strains isolated from patients and healthy individuals.
A total of 90 non-duplicate Enterococcus spp isolates were isolated from patients and healthy individuals. Antibiotic susceptibility pattern was determined by disk diffusion and E-test method. Virulence genes and two species of enterococci were determined by PCR amplification. The capacity of biofilm formation was also evaluated by microtiter plate technique.
E. faecalis was the predominant species among our clinical isolates (80%). The prevalence of agg and ace genes was 37.8% and 73.3% in clinical and 8.9% and 11.1% in "healthy" samples, respectively. The rate of Multiple Drug Resistant strains was 73.3% and 11.1% in clinical and "healthy" isolates, respectively. The ability of biofilm formation was significantly higher in clinical compared to "healthy" isolates (100% vs 75.6%, P < 0.05).
The frequency of ace and agg genes, antibiotic resistance and biofilm formation ability were significantly higher in clinical than in "healthy" isolates (P < 0.05). Existence of agg and ace genes, biofilm formation and antibiotic resistance among the healthy enterococci isolates has a special importance since, in case these strains spread through clinical environments or reach water sources, this issue can be considered as a risk factor for health and sanitation of societY.
本研究旨在确定并比较从患者和健康个体中分离出的肠球菌属菌株的抗生素耐药谱、生物膜形成能力以及agg和ace基因的频率。
从患者和健康个体中总共分离出90株非重复的肠球菌属菌株。通过纸片扩散法和E试验法确定抗生素敏感性模式。通过PCR扩增确定毒力基因和两种肠球菌。还通过微量滴定板技术评估生物膜形成能力。
粪肠球菌是我们临床分离株中的主要菌种（80%）。临床样本中agg和ace基因的流行率分别为37.8%和73.3%，“健康”样本中分别为8.9%和11.1%。临床分离株和“健康”分离株中多重耐药菌株的比例分别为73.3%和11.1%。与“健康”分离株相比，临床分离株的生物膜形成能力显著更高（100%对75.6%，P<0.05）。
临床分离株中ace和agg基因的频率、抗生素耐药性和生物膜形成能力显著高于“健康”分离株（P<0.05）。健康肠球菌分离株中存在agg和ace基因、生物膜形成和抗生素耐药性具有特殊重要性，因为如果这些菌株在临床环境中传播或进入水源，这一问题可被视为社会健康和卫生的一个危险因素。
