Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Food Res Int. 2019 Feb;116:258-265. doi: 10.1016/j.foodres.2018.08.030. Epub 2018 Aug 13.
Cronobacter sakazakii (Cs) is a typical foodborne bacterium that infect powdered infant formula (PIF) worldwide. In this study, a recombinant antimicrobial peptide, branded as Funme peptide (FP)was applied to protect PIF from Cs contamination. The result from the antimicrobial activity assay showed that the minimum inhibitory concentration (MIC) of BMAP-27 peptide, FP and Ampicillin against Cs were 250.0, 125.0 and 15.6 μg/mL, respectively, indicating FP possessed higher MIC than that of Ampicillin, and lower MIC than that of BMAP-27. The minimum biofilm eradication concentration (MBEC) assay showed that FP at 2 × MIC (250.0 μg/mL) could completely eradicated Cs biofilms. The antibacterial activity of FP might be due to the increasing permeability and the release of cytoplasmic β-galactosidase of Cs. The results acquired from transmission electron microscopy and scanning electron microscopy indicated that FP induced the disruption and dysfunction of cell walls and membranes. Moreover, safety assay showed that FP had low cytotoxicity to human erythrocytes. The present study investigated the antibacterial effects and mechanisms of FP against Cs, providing promising evidence to apply this novel antimicrobial agent against Cs contamination in foods and food processing facilities.
阪崎克罗诺杆菌（Cs）是一种典型的食源性细菌，在全球范围内感染粉状婴儿配方食品（PIF）。在本研究中，一种重组抗菌肽，命名为 Funme 肽（FP），被应用于保护 PIF 免受 Cs 污染。抗菌活性测定结果表明，BMAP-27 肽、FP 和氨苄西林对 Cs 的最小抑菌浓度（MIC）分别为 250.0、125.0 和 15.6μg/mL，表明 FP 的 MIC 高于氨苄西林，低于 BMAP-27。最低生物膜清除浓度（MBEC）测定表明，FP 在 2×MIC（250.0μg/mL）时可完全清除 Cs 生物膜。FP 的抗菌活性可能是由于 Cs 的通透性增加和细胞质β-半乳糖苷酶的释放。透射电子显微镜和扫描电子显微镜的结果表明，FP 诱导了细胞壁和细胞膜的破坏和功能障碍。此外，安全性测定表明，FP 对人红细胞的细胞毒性较低。本研究探讨了 FP 对 Cs 的抗菌作用及其机制，为在食品和食品加工设施中应用这种新型抗菌剂对抗 Cs 污染提供了有希望的证据。
