Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Beijing 100193, People's Republic of China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Food Res Int. 2019 Feb;116:878-882. doi: 10.1016/j.foodres.2018.09.023. Epub 2018 Sep 10.
Abscisic acid (ABA), as a sesquiterpenoid hormone, could regulate lots of physiological processes, especially secondary metabolism in plants. Nevertheless, its mechanism of action, from the perspective of protein expression, remains largely unknown. In the study, isobaric tags for relative and absolute quantitation (iTRAQ) was employed to investigate ABA treatment-induced proteomic changes related to secondary metabolism in soybean sprouts. Among the 3033 proteins identified, compared with the control, ABA treatment up- and down-regulated 350 proteins. These proteins were involved in GABA biosynthesis, such as glutamate synthase, glutamate decarboxylase (GAD), methionine synthetase, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1, aminoaldehyde dehydrogenase (AMADH) and inositol phosphate metabolism pathways, including phosphoinositide phospholipase C (PI-PLC), purple acid phosphatase (PAP) and inositol polyphosphate 5-phosphatase. In addition, flavonoid biosynthetic proteins, such as cinnamate 4-hydroxylase, chalcone isomerase, chalcone synthase, isoflavone synthase and isoflavone reductase, were also modulated in response to ABA treatment. What's more, ABA treatment regulated proteins involved in ABA signal transduction, such as SNF1-related protein kinase (SnRK), protein phosphatase 2C (PP2C), guanine nucleotide-binding protein and calreticulin-3.
脱落酸（ABA）作为一种倍半萜激素，可以调节许多生理过程，特别是植物的次生代谢。然而，其作用机制，从蛋白质表达的角度来看，仍然很大程度上未知。在这项研究中，我们使用了相对和绝对定量同位素标签（iTRAQ）技术来研究 ABA 处理诱导的与大豆芽次生代谢相关的蛋白质组学变化。在鉴定的 3033 种蛋白质中，与对照相比，ABA 处理上调和下调了 350 种蛋白质。这些蛋白质参与 GABA 生物合成，如谷氨酸合酶、谷氨酸脱羧酶（GAD）、甲硫氨酸合成酶、5-甲基四氢叶酸-同型半胱氨酸甲基转移酶 1、氨基酸醛脱氢酶（AMADH）和肌醇磷酸盐代谢途径，包括磷酸肌醇磷脂酶 C（PI-PLC）、酸性磷酸酶（PAP）和肌醇多磷酸盐 5-磷酸酶。此外，类黄酮生物合成蛋白，如肉桂酸 4-羟化酶、查尔酮异构酶、查尔酮合酶、异黄酮合酶和异黄酮还原酶，也对 ABA 处理作出了响应。更重要的是，ABA 处理调节了 ABA 信号转导相关的蛋白质，如 SNF1 相关蛋白激酶（SnRK）、蛋白磷酸酶 2C（PP2C）、鸟苷酸结合蛋白和钙网蛋白-3。
