Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Food Chem. 2019 Mar 1;275:467-473. doi: 10.1016/j.foodchem.2018.09.064. Epub 2018 Sep 12.
It has been shown that 15 μW·cm UV-B radiation has the most pronounced effects on γ-aminobutiric acid (GABA), inositol 1,4,5-trisphosphate (IP3) and abscisic acid (ABA) accumulation in 4-day-old soybean sprouts. Nevertheless, its mechanism of action, from the perspective of protein expression, remains largely unknown. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) were employed to investigate UV-B treatment-induced proteomic changes in soybean sprouts. Results showed that UV-B treatment effectively regulated proteins involved in GABA biosynthesis, such as glutamate synthase, glutamate decarboxylase (GAD), methionine synthetase, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, aminoaldehyde dehydrogenase (AMADH) and inositol phosphate metabolism pathways, including phosphoinositide phospholipase C (PI-PLC), purple acid phosphatase (PAP) and inositol polyphosphate 5-phosphatase. In addition, proteins involved in ABA biosynthesis and signal transduction, such as 9-cis-epoxycarotenoid dioxygenase (NCED), abscisic-aldehyde oxidase (AO), SNF1-related protein kinase (SnRK), protein phosphatase 2C (PP2C), guanine nucleotide-binding protein and calreticulin-3, were also modulated under UV-B treatment.
已经表明，15μW·cm 的 UV-B 辐射对 4 天大的大豆芽中γ-氨基丁酸（GABA）、肌醇 1,4,5-三磷酸（IP3）和脱落酸（ABA）的积累有最显著的影响。然而，其作用机制，从蛋白质表达的角度来看，在很大程度上仍然未知。在这项研究中，采用相对和绝对定量标记（iTRAQ）来研究 UV-B 处理诱导的大豆芽的蛋白质组变化。结果表明，UV-B 处理有效地调节了 GABA 生物合成相关的蛋白质，如谷氨酸合酶、谷氨酸脱羧酶（GAD）、蛋氨酸合成酶、5-甲基四氢叶酸-同型半胱氨酸甲基转移酶、氨基酸醛脱氢酶（AMADH）和肌醇磷酸盐代谢途径，包括磷酸肌醇磷脂酶 C（PI-PLC）、紫色酸性磷酸酶（PAP）和肌醇多磷酸盐 5-磷酸酶。此外，ABA 生物合成和信号转导相关的蛋白质，如 9-顺式-环氧类胡萝卜素双加氧酶（NCED）、脱落酸醛氧化酶（AO）、SNF1 相关蛋白激酶（SnRK）、蛋白磷酸酶 2C（PP2C）、鸟嘌呤核苷酸结合蛋白和钙网蛋白-3，也在 UV-B 处理下被调节。
