Jeong Hyo-Geun, Han Yoon-Soo, Jung Kyung-Hye, Kim Young-Jin
Department of Biomedical Engineering, Daegu Catholic University, Gyeongsan 38430, Korea.
Department of Advanced Materials and Chemical Engineering, Daegu Catholic University, Gyeongsan 38430, Korea.
Nanomaterials (Basel). 2019 Feb 1;9(2):184. doi: 10.3390/nano9020184.
To provide adequate conditions for the regeneration of damaged bone, it is necessary to develop piezoelectric porous membranes with antioxidant and anti-inflammatory activities. In this study, composite nanofibers comprising poly(vinylidene fluoride) (PVDF) and a polyhedral oligomeric silsesquioxane⁻epigallocatechin gallate (POSS⁻EGCG) conjugate were fabricated by electrospinning methods. The resulting composite nanofibers showed three-dimensionally interconnected porous structures. Their average diameters, ranging from 936 ± 223 nm to 1094 ± 394 nm, were hardly affected by the addition of the POSS⁻EGCG conjugate. On the other hand, the piezoelectric β-phase increased significantly from 77.4% to 88.1% after adding the POSS⁻EGCG conjugate. The mechanical strength of the composite nanofibers was ameliorated by the addition of the POSS⁻EGCG conjugate. The results of in vitro bioactivity tests exhibited that the proliferation and differentiation of osteoblasts (MC3T3-E1) on the nanofibers increased with the content of POSS⁻EGCG conjugate because of the improved piezoelectricity and antioxidant and anti-inflammatory properties of the nanofibers. All results could suggest that the PVDF composite nanofibers were effective for guided bone regeneration.
为受损骨骼的再生提供充足条件，开发具有抗氧化和抗炎活性的压电多孔膜很有必要。在本研究中，通过静电纺丝法制备了包含聚偏二氟乙烯（PVDF）和多面体低聚倍半硅氧烷⁻表没食子儿茶素没食子酸酯（POSS⁻EGCG）共轭物的复合纳米纤维。所得复合纳米纤维呈现三维相互连接的多孔结构。它们的平均直径在936±223纳米至1094±394纳米之间，添加POSS⁻EGCG共轭物几乎不影响其平均直径。另一方面，添加POSS⁻EGCG共轭物后，压电β相从77.4%显著增加到88.1%。添加POSS⁻EGCG共轭物改善了复合纳米纤维的机械强度。体外生物活性测试结果表明，由于纳米纤维压电性、抗氧化和抗炎性能的改善，纳米纤维上成骨细胞（MC3T3-E1）的增殖和分化随POSS⁻EGCG共轭物含量的增加而增加。所有结果表明，PVDF复合纳米纤维对引导骨再生有效。
