Institute of Biochemical Plant Physiology and Bioeconomy Science Center (BioSC), Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Sci Rep. 2019 Feb 4;9(1):1287. doi: 10.1038/s41598-018-37571-x.
The plant hormone ethylene was identified as important triggering factor and primary regulator of flower senescence in many species. Consequently, application of chemical inhibitors of ethylene biosynthesis and action is used to extend the longevity of ethylene-sensitive flowers. Here, we show that the peptide NOP-1, a biological derived from the nuclear localization signal of ethylene regulator EIN2 tightly binds to the ethylene receptor of carnation plants - a model to study flower senescence. When applied on cut flowers the peptide biological delays petal senescence similar to previously identified and currently used chemical inhibitors, but offers significant advances to these chemicals in biodegradability, sustainability and ecotoxicity. Our bioinformatic analysis of a wide range of ethylene receptors indicates complete sequence conservation of the anticipated NOP-1 binding site in flower species supporting a widespread use of the peptide on flowering ornamentals to delay senescence and decay in cut flowers. We anticipate our innovative approach to extend flower longevity by a new class of biomolecules such as peptides, peptide analogues and peptide mimetics will significantly advance our technological capability to delay flower senescence and expand vase-life of cut flowers in a sustainable and environmentally friendly manner.
植物激素乙烯被确定为许多物种中花朵衰老的重要触发因素和主要调节因子。因此，应用化学抑制剂来抑制乙烯的生物合成和作用被用来延长乙烯敏感花朵的寿命。在这里，我们展示了一种肽 NOP-1，它是从乙烯调节因子 EIN2 的核定位信号衍生而来的生物，它与康乃馨植物的乙烯受体紧密结合——这是研究花朵衰老的模型。当应用于切花时，该肽生物类似于先前鉴定和当前使用的化学抑制剂一样，延迟花瓣衰老，但在生物降解性、可持续性和生态毒性方面，比这些化学物质有显著的优势。我们对广泛的乙烯受体进行生物信息学分析表明，在支持在开花观赏植物上广泛应用该肽以延迟衰老和切花腐烂的花卉物种中，预期的 NOP-1 结合位点的序列完全保守。我们预计，通过一类新的生物分子（如肽、肽类似物和肽模拟物）来延长花期的创新方法，将显著提高我们以可持续和环保的方式延迟花朵衰老和延长切花瓶寿命的技术能力。
