Sarma Rupam, Islam Md Saiful, Running Mark P, Bhattacharyya Dibakar
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506.
Department of Biology, University of Louisville, Louisville, KY 40292.
Polymers (Basel). 2018 Apr;10(4). doi: 10.3390/polym10040463. Epub 2018 Apr 23.
We have developed a multienzyme functionalized membrane reactor for bioconversion of lignin model compound involving enzymatic catalysis. Layer-by-layer approach was used to immobilize three different enzymes (glucose oxidase, peroxidase and laccase) into pH-responsive membranes. This novel membrane reactor couples the in situ generation of hydrogen peroxide (by glucose oxidase) to oxidative conversion of a lignin model compound, guaiacylglycerol-B-guaiacylether (GGE). Preliminary investigation of the efficacy of these functional membranes towards GGE degradation is demonstrated under convective flow mode. Over 90% of the initial feed could be degraded with the multienzyme immobilized membranes at a residence time of approximately 22 seconds. GGE conversion product analysis revealed formation of oligomeric oxidation products with peroxidase, which might be potential hazard to membrane bioreactors. These oxidation products could be further degraded by laccase enzymes in the multienzymatic membranes explaining the potential of multienzyme membrane reactors. The multienzyme incorporated membrane reactors were active for about a month time of storage at 4 °C, and retention of activity was demonstrated after repetitive use.
我们开发了一种用于木质素模型化合物生物转化的多酶功能化膜反应器，该过程涉及酶催化。采用层层组装法将三种不同的酶（葡萄糖氧化酶、过氧化物酶和漆酶）固定在pH响应膜中。这种新型膜反应器将过氧化氢的原位生成（通过葡萄糖氧化酶）与木质素模型化合物愈创木基甘油-β-愈创木基醚（GGE）的氧化转化相结合。在对流流动模式下对这些功能膜降解GGE的效果进行了初步研究。在约22秒的停留时间内，固定有多酶的膜可降解超过90%的初始进料。GGE转化产物分析表明，过氧化物酶会形成低聚氧化产物，这可能对膜生物反应器构成潜在危害。这些氧化产物可被多酶膜中的漆酶进一步降解，这说明了多酶膜反应器的潜力。含有多酶的膜反应器在4℃下储存约一个月仍具有活性，并且在重复使用后仍保持活性。
