Rashid Mohammed H, Vaughan Jane L, Stevenson Mark A, Campbell Angus J D, Saeed Muhammad A, Indjein Léa, Beveridge Ian, Jabbar Abdul
Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Werribee, Victoria 3030, Australia.
Cria Genesis, PO Box 406, Ocean Grove, Victoria, 3226, Australia.
Parasitol Res. 2019 Mar;118(3):891-900. doi: 10.1007/s00436-019-06235-8. Epub 2019 Feb 4.
This study involved a national cross-sectional survey of gastrointestinal nematodes (GINs) of alpacas in Australia. A total of 1545 fresh faecal samples were collected from both sexes of alpacas and processed for faecal egg counts (FEC) and molecular identification of nematodes using the multiplexed tandem PCR assay. Based on egg morphology, the overall prevalence of GINs was 66% while that for strongyles was 59%. The overall mean FEC was 276 eggs per gram (EPG) of faeces, with the highest count of 17,415 EPG. Male alpacas had a higher prevalence (68%, 334/490) as well as mean FEC (328 ± 60 EPG) of GINs than females (63%, 602/954; 227 ± 26, respectively). Weaners had the highest prevalence (80%) whereas tuis had the highest FEC (402 EPG) of nematodes. The highest prevalence (77%, 293/383) and FEC (630 EPG) of GINs were observed in the summer rainfall zone followed by the Mediterranean-type rainfall, non-seasonal rainfall and winter rainfall zones. The characterisation of nematode DNA isolated from faeces revealed the occurrence of seven different GINs, including Camelostrongylus mentulatus, Cooperia spp., Haemonchus spp., Oesophagostomum spp., Ostertagia ostertagi, Teladorsagia circumcincta and Trichostrongylus spp. Besides, Nematodirus spp. and Trichuris spp. were also found during FECs. The prevalence of Haemonchus spp. was highest in the summer rainfall zone while that of C. mentulatus was highest in the Mediterranean-type rainfall, non-seasonal rainfall and winter rainfall zones. The findings of this study revealed that alpacas harbour many of the same nematodes as sheep and cattle.
本研究对澳大利亚羊驼胃肠道线虫（GINs）进行了全国性横断面调查。共从羊驼的雌雄个体中采集了1545份新鲜粪便样本，并对其进行粪便虫卵计数（FEC）处理，同时使用多重串联PCR检测法对线虫进行分子鉴定。根据虫卵形态，GINs的总体感染率为66%，而圆线虫的感染率为59%。粪便虫卵总体平均计数为每克粪便276个虫卵（EPG），最高计数为17415 EPG。雄性羊驼的GINs感染率（68%，334/490）和平均FEC（328±60 EPG）均高于雌性羊驼（分别为63%，602/954；227±26）。断奶羊驼的线虫感染率最高（80%），而成年羊驼的FEC最高（402 EPG）。在夏季降雨区观察到GINs的最高感染率（77%，293/383）和FEC（630 EPG），其次是地中海型降雨区、非季节性降雨区和冬季降雨区。从粪便中分离出的线虫DNA特征显示存在七种不同的GINs，包括有钩骆驼线虫、古柏属线虫、血矛线虫属、食道口线虫属、奥氏奥斯特线虫、环形泰勒线虫和毛圆线虫属。此外，在FEC检测过程中还发现了细颈线虫属和鞭虫属。血矛线虫属的感染率在夏季降雨区最高，而有钩骆驼线虫在 地中海型降雨区、非季节性降雨区和冬季降雨区的感染率最高。本研究结果表明，羊驼体内携带的许多线虫与绵羊和牛相同。
