Rashid Mohammed H, Beveridge Ian, Vaughan Jane L, Jabbar Abdul
Department of Veterinary Biosciences, Melbourne School of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia.
Cria Genesis, PO Box 406, Ocean Grove, Victoria, 3226, Australia.
Parasitol Res. 2019 Mar;118(3):1031-1038. doi: 10.1007/s00436-019-06237-6. Epub 2019 Feb 8.
In this study, 100 gastrointestinal tracts of Australian alpacas were examined to assess the worm burden and to identify the species of nematode present. Faecal samples were collected from 97 alpacas and processed for faecal egg counts (FECs). For identification of the species, both molecular (multiplexed-tandem polymerase chain reaction [MT-PCR]) and morphological techniques were used. Total worm counts (TWCs) revealed a mean burden of 1300 worms, with the highest burden of 29,000 worms. The average egg count was 501 eggs per gram of faeces (EPG), with the highest count of 3500 EPG. Nineteen different species of gastrointestinal nematodes (GINs) were identified, and Graphinema auchenia, Camelostrongylus mentulatus and Trichuris tenuis were recovered from Australian alpacas for the first time. Haemonchus contortus was the most prevalent nematode (81%) followed by C. mentulatus (60%). The majority of the nematodes found are shared with sheep, goats and cattle. Findings of this study provide useful insights into the spectrum of GINs and their burden in Australian alpacas.
在本研究中，对100只澳大利亚羊驼的胃肠道进行了检查，以评估蠕虫负荷并确定存在的线虫种类。从97只羊驼收集粪便样本并进行粪便虫卵计数（FEC）处理。为了鉴定线虫种类，同时使用了分子技术（多重串联聚合酶链反应[MT-PCR]）和形态学技术。总蠕虫计数（TWC）显示平均负荷为1300条蠕虫，最高负荷为29,000条蠕虫。平均每克粪便虫卵数（EPG）为501个，最高计数为3500 EPG。鉴定出19种不同的胃肠道线虫（GIN），首次从澳大利亚羊驼体内发现了奥氏格拉线虫、有钩似细颈线虫和鞭虫。捻转血矛线虫是最常见的线虫（81%），其次是有钩似细颈线虫（60%）。发现的大多数线虫与绵羊､山羊和牛相同。本研究结果为澳大利亚羊驼胃肠道线虫谱及其负荷提供了有用的见解。
