Department of Obstetrics, Baoan Maternal and Child Health Hospital, Jinan University, Shenzhen, Guangdong 518102, P.R. China.
Department of Obstetrics and Gynecology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong 518100, P.R. China.
Mol Med Rep. 2019 Apr;19(4):2463-2470. doi: 10.3892/mmr.2019.9911. Epub 2019 Jan 30.
Differential proteomic technology was used to identify urine proteomic profile of gestational hypertension and preeclampsia. Urine samples were collected from 10 patients with gestational hypertension, 10 patients with mild preeclampsia, 10 patients with severe preeclampsia and 10 normal pregnancies and analyzed by 2‑D difference gel electrophoresis, then matrix assisted laser desorption ionization mass spectrometry was used to identify differential proteins. Subsequently, ELISA was used to verify the content variation of the identified proteins in 200 urine samples. In total, 30 differential proteins were identified. For prostaglandin‑H2 D‑isomerase (L‑PGDS), perlecan and other 15 proteins, the contents in patients with gestational hypertension were higher than that of normal pregnancies, but lower in mild and severe preeclampsia. By contrast, serum albumin and α‑1‑antitrypsin was lower in samples from patients with gestational hypertension and higher in patients with mild and severe preeclampsia compared with normal pregnancies. ELISA verified that the urinary concentration of L‑PGDS and perlecan were significantly lower in patients with preeclampsia than in normal pregnancies (P<0.05). Urine proteomics is a useful tool to identify potential biomarkers to distinguish between different types of hypertensive disorders in pregnancy. L‑PGDS and perlecan could potentially be used as markers to reflect the state of renal function, and may participate in the genesis and development of renal injury during preeclampsia.
采用差异蛋白质组学技术鉴定妊娠期高血压和子痫前期的尿蛋白质组特征。收集 10 例妊娠期高血压患者、10 例轻度子痫前期患者、10 例重度子痫前期患者和 10 例正常妊娠孕妇的尿液标本，采用 2-DE 差异凝胶电泳进行分析，然后采用基质辅助激光解吸电离飞行时间质谱技术鉴定差异蛋白质，接着采用 ELISA 法验证 200 例尿样中鉴定出的蛋白质含量变化。共鉴定出 30 个差异蛋白质。对于前列腺素 H2 D-异构酶（L-PGDS）、硫酸乙酰肝素蛋白聚糖和其他 15 种蛋白质，妊娠期高血压患者中的含量高于正常妊娠孕妇，但轻度和重度子痫前期患者中的含量较低。相比之下，血清白蛋白和α-1-抗胰蛋白酶在妊娠期高血压患者的样本中含量较低，在轻度和重度子痫前期患者中的含量较高。ELISA 验证子痫前期患者的尿液 L-PGDS 和硫酸乙酰肝素蛋白聚糖浓度明显低于正常妊娠孕妇（P<0.05）。尿蛋白质组学是一种有用的工具，可用于鉴定潜在的生物标志物，以区分不同类型的妊娠期高血压疾病。L-PGDS 和硫酸乙酰肝素蛋白聚糖可能可作为反映肾功能状态的标志物，可能参与子痫前期肾损伤的发生和发展。
