Department of Surgery, Zhangqiu District Maternal and Child Health Care Hospital, Jinan, Shandong, China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):598-603. doi: 10.26355/eurrev_201901_16872.
To investigate the clinicopathological significance of long non-coding LINC00958 and its expression in gastric cancer (GC).
A total of 200 patients with GC whose sample tissues were enrolled. Total RNA was isolated using the TRIzol method followed by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) detection of LINC00958 on mRNA level. The correlation was analyzed using Chi-square test, between the LINC00958 expression versus clinicopathological variables, including age, gender, clinical stage, tumor size, TNM classification and overall prognosis. The Kaplan-Meier survival curve was used to assess the prognostic value of LINC00958 expression, after which univariate and multivariate analysis was carried out with the COX proportional hazard analysis.
LINC00958 was shown to be dramatically elevated in GC relative to normal controls. Elevated LINC00958 significantly correlated with lymph nodes metastases, distance metastasis, peritoneal dissemination, and peritumoral tissues infiltration (p<0.05). The Kaplan-Meier survival analysis showed that up-regulated LINC00958 was markedly associated with inferior overall survival (p<0.001). In addition, multivariate analysis highlighted that LINC00958 expression was an independent prognostic factor in GC.
Our observation revealed that elevated LINC00958 was significantly associated with metastasis and was an independent prognostic factor in GC, indicating that LINC00958 can serve as a novel prognostic predictor in GC.
研究长链非编码 LINC00958 在胃癌（GC）中的临床病理意义及其表达。
共纳入 200 例 GC 患者的样本组织。采用 TRIzol 法提取总 RNA，采用定量实时聚合酶链反应（qRT-PCR）检测 LINC00958 在 mRNA 水平上的表达。采用卡方检验分析 LINC00958 表达与临床病理变量之间的相关性，包括年龄、性别、临床分期、肿瘤大小、TNM 分类和总体预后。采用 Kaplan-Meier 生存曲线评估 LINC00958 表达的预后价值，然后采用 COX 比例风险分析进行单因素和多因素分析。
LINC00958 在 GC 中明显升高，与正常对照组相比。LINC00958 的升高与淋巴结转移、远处转移、腹膜扩散和肿瘤周围组织浸润显著相关（p<0.05）。Kaplan-Meier 生存分析显示，上调的 LINC00958 与总体生存率明显降低显著相关（p<0.001）。此外，多因素分析强调，LINC00958 表达是 GC 的独立预后因素。
我们的观察结果表明，LINC00958 的升高与转移显著相关，是 GC 的独立预后因素，表明 LINC00958 可以作为 GC 的一种新的预后预测因子。
