LINC00958 通过靶向 miR-490-3p 和 AURKA 促进膀胱癌发生。

LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA.

机构信息

Department of Urology Ward 1, Zhengzhou Central Hospital Affiliated to Zhengzhou University, No. 195 Tongbai Road, Zhongyuan District, Zhengzhou, 450007, Henan, China.

Department of Urology, Peking University Cancer Hospital, Beijing, 100142, China.

出版信息

BMC Cancer. 2021 Oct 26;21(1):1145. doi: 10.1186/s12885-021-08882-6.

BACKGROUND

Bladder cancer is a prevalent malignancy of the urinary system, in which long non-coding RNAs (lncRNAs) are highly associated. We aimed to elucidate the role of LINC00958 in bladder cancer.

METHODS

LINC00958 expression levels were measured using qRT-PCR. The interaction of LINC00958-miR-490-3p-AURKA was analyzed by luciferase, RIP, and RNA pull-down assays. The biological roles of LINC00958, miR-490-3p, and AURKA in bladder cancer cells were analyzed using CCK8, BrdU, and transwell assays.

RESULTS

Increased expression of LINC00958 and AURKA was observed in bladder cancer tissues and cell lines. Decreased LINC00958 expression repressed bladder cancer progression and downregulation of miR-490-3p accelerated bladder cancer cell progression. Moreover, LINC00958 sponges miR-490-3p to upregulate AURKA expression, thereby promoting carcinogenesis in bladder cancer cells.

CONCLUSIONS

Our study revealed that LINC00958 facilitated cell proliferation and invasion, and suppressed cell apoptosis by sponging miR-490-3p and upregulating AURKA, thus inspiring a new treatment method for bladder cancer.

背景

膀胱癌是泌尿系统常见的恶性肿瘤，其中长链非编码 RNA（lncRNA）高度相关。我们旨在阐明 LINC00958 在膀胱癌中的作用。

方法

使用 qRT-PCR 测量 LINC00958 的表达水平。通过荧光素酶、RIP 和 RNA 下拉测定分析 LINC00958-miR-490-3p-AURKA 的相互作用。使用 CCK8、BrdU 和 Transwell 测定分析 LINC00958、miR-490-3p 和 AURKA 在膀胱癌细胞中的生物学作用。