1 Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
Technol Cancer Res Treat. 2019 Jan-Dec;18:1533033819852990. doi: 10.1177/1533033819852990.
Alterations in microRNAs expression can accelerate the development of human cancers. However, the role of miR-153-3p in acute lymphoblastic leukemia remains unknown. The expression of miR-153-3p in acute lymphoblastic leukemia cell lines was measured by quantitative real-time polymerase chain reaction. Effects of miR-153-3p expression on acute lymphoblastic leukemia cell proliferation, migration, and invasion were examined by Cell Counting Kit-8 assay, wound healing assay, and Transwell invasion assay, respectively. We then validated inhibitor of growth protein 2 as a direct target of miR-153-3p through bioinformatics analysis, luciferase activity reporter assay, and Western blot assay. The miR-153-3p expression was decreased in acute lymphoblastic leukemia cell lines. Cell proliferation, migration, and invasion of acute lymphoblastic leukemia were obviously decreased by miR-153-3p overexpression. Moreover, inhibitor of growth protein 2 was validated as a direct target of miR-153-3p and the overexpression of inhibitor of growth protein 2 reversed the suppressive effects of miR-153-3p on acute lymphoblastic leukemia cell behaviors. Based on these results, we provided evidence that miR-153-3p might be a target for the treatment of acute lymphoblastic leukemia.
miRNAs 表达的改变可加速人类癌症的发展。然而，miR-153-3p 在急性淋巴细胞白血病中的作用尚不清楚。通过实时定量聚合酶链反应测量急性淋巴细胞白血病细胞系中 miR-153-3p 的表达。通过细胞计数试剂盒-8 测定、划痕愈合测定和 Transwell 侵袭测定分别检测 miR-153-3p 表达对急性淋巴细胞白血病细胞增殖、迁移和侵袭的影响。然后通过生物信息学分析、荧光素酶活性报告测定和 Western blot 测定验证生长抑制蛋白 2 是 miR-153-3p 的直接靶标。miR-153-3p 在急性淋巴细胞白血病细胞系中的表达降低。miR-153-3p 过表达明显降低了急性淋巴细胞白血病的细胞增殖、迁移和侵袭。此外，生长抑制蛋白 2 被验证为 miR-153-3p 的直接靶标，生长抑制蛋白 2 的过表达逆转了 miR-153-3p 对急性淋巴细胞白血病细胞行为的抑制作用。基于这些结果，我们提供了证据表明 miR-153-3p 可能是治疗急性淋巴细胞白血病的靶点。
