Dong Wenjing, Han Xinyue, Bao Chao, Tai Saijilahu, Bai Yuxia, Xu Liang, Yang Jingfeng, Leung TinChung, Ao Wuliji, Dong Wu
Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicant and Toxicology, College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia 028000, China.
Mongolian State University of Education, Ulaanbaatar 210648, Mongolia.
Evid Based Complement Alternat Med. 2021 Apr 26;2021:5543259. doi: 10.1155/2021/5543259. eCollection 2021.
Melanoma is a serious malignant form of skin cancer. Euphorbiaceae compound B (ECB, 2,4-dihydroxy-6-methoxy-3-methylacetophenone) is an acetophenone compound that is isolated from Hayata (EEH), an herbaceous perennial, and has antitumor activity. Here, we transplanted human melanoma cells into zebrafish embryos to establish a zebrafish/melanoma model. We showed that this model can be used to evaluate the therapeutic effect of EEH and ECB and discussed its potential mechanism of action. The results showed that ECB was an active ingredient of EEH in inhibiting melanoma-induced hyperplasia of blood vessels in zebrafish embryos, similar to the angiogenic inhibitor vatalanib. ECB inhibited the number and length of subintestinal veins ( < 0.05), as well as the distribution of melanoma in zebrafish embryos ( < 0.05). More importantly, unlike vatalanib, ECB only inhibited melanoma-induced abnormal and excessive growth of blood vessels in xenografts. In addition, ECB inhibited the mRNA expression of and in zebrafish. Both and are essential genes that regulate blood vessel formation and upregulate the expression of and genes in zebrafish. Together, the above-mentioned results indicate that ECB has a potential antimelanoma effect , which may be mediated by inhibiting vascular endothelial growth factor receptors.
黑色素瘤是一种严重的皮肤癌恶性形式。大戟科化合物B（ECB，2,4 - 二羟基 - 6 - 甲氧基 - 3 - 甲基苯乙酮）是一种从多年生草本植物台湾泽漆（EEH）中分离出的苯乙酮类化合物，具有抗肿瘤活性。在此，我们将人黑色素瘤细胞移植到斑马鱼胚胎中，建立了斑马鱼/黑色素瘤模型。我们证明该模型可用于评估EEH和ECB的治疗效果，并探讨其潜在作用机制。结果表明，ECB是EEH抑制斑马鱼胚胎中黑色素瘤诱导的血管增生的活性成分，类似于血管生成抑制剂凡他尼布。ECB抑制了斑马鱼胚胎中肠下静脉的数量和长度（<0.05），以及黑色素瘤在斑马鱼胚胎中的分布（<0.05）。更重要的是，与凡他尼布不同，ECB仅抑制异种移植中黑色素瘤诱导的血管异常和过度生长。此外，ECB抑制了斑马鱼中vegfr1和vegfr2的mRNA表达。vegfr1和vegfr2都是调节血管形成的必需基因，并上调斑马鱼中flk1和flt4基因的表达。综上所述，上述结果表明ECB具有潜在的抗黑色素瘤作用，这可能是通过抑制血管内皮生长因子受体介导的。
