Wei Taotao, Dai Gaozhong, Liu Tianye, Tian Yaozhou
Wuxi hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, China.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Front Cell Infect Microbiol. 2025 Jul 18;15:1517751. doi: 10.3389/fcimb.2025.1517751. eCollection 2025.
Previous studies have established a relationship between duodenal mucosa-associated microbiota and overall health. However, sampling duodenal microbiota is technically challenging. Mucosal biopsies collected via endoscopy are the most common approach, but this method risks contamination of the working channel with gastrointestinal contents or extraneous microorganisms.
This study designed a novel accessory, an endoscopic channel plug, to improve the sampling process by ensuring a clean and sterile working channel, thereby providing more accurate microbiota results.
Microbiome analysis of samples collected from the oral cavity, traditional duodenal sampling, and the modified method with the channel plug revealed that samples obtained with the plug exhibited higher PCR product concentrations and a greater number of operational taxonomic units (335). Additionally, 16S rRNA sequencing showed significant differences in the taxonomic composition at both the phylum and genus levels among the different sampling methods. Notably, the novel method group (using the channel plug) contained a higher abundance of , whereas this genus was less abundant in oral cavity and traditional duodenal samples. Furthermore, the abundance of specific bacterial strains varied significantly between sampling methods. These findings suggest that the use of the channel plug enables more comprehensive microbiota sampling, providing data to support clinical diagnosis of gastrointestinal diseases.
先前的研究已经确立了十二指肠黏膜相关微生物群与整体健康之间的关系。然而，采集十二指肠微生物群在技术上具有挑战性。通过内镜检查采集的黏膜活检样本是最常见的方法，但这种方法存在工作通道被胃肠道内容物或外来微生物污染的风险。
本研究设计了一种新型附件——内镜通道塞，通过确保工作通道清洁无菌来改进采样过程，从而提供更准确的微生物群结果。
对从口腔、传统十二指肠采样以及使用通道塞的改良方法采集的样本进行微生物组分析发现，使用通道塞获得的样本显示出更高的PCR产物浓度和更多的可操作分类单元（335个）。此外，16S rRNA测序显示不同采样方法在门和属水平的分类组成上存在显著差异。值得注意的是，新型方法组（使用通道塞）中某属的丰度较高，而该属在口腔和传统十二指肠样本中的丰度较低。此外，特定细菌菌株的丰度在不同采样方法之间差异显著。这些发现表明，使用通道塞能够实现更全面的微生物群采样，为胃肠道疾病的临床诊断提供数据支持。
