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DNA-dependent RNA polymerase of Escherichia coli induces bending or an increased flexibility of DNA by specific complex formation.

作者信息

Heumann H, Ricchetti M, Werel W

机构信息

Max-Planck-Institut für Biochemie, Martinsried, FRG.

出版信息

EMBO J. 1988 Dec 20;7(13):4379-81. doi: 10.1002/j.1460-2075.1988.tb03336.x.

DOI:10.1002/j.1460-2075.1988.tb03336.x
PMID:3072199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC455170/
Abstract

Escherichia coli RNA polymerase is shown to induce bending or an increased flexibility of the promoter DNA. This is a specific effect of holoenzyme (core enzyme and sigma-factor). The centre of the flexibility is 3 bp upstream of the initiation point of RNA synthesis. This flexibility or bending is maintained during RNA synthesis by core enzyme.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b8/455170/dd901224bfc1/emboj00150-0348-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b8/455170/c262f45fc5ca/emboj00150-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b8/455170/dd901224bfc1/emboj00150-0348-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b8/455170/c262f45fc5ca/emboj00150-0348-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b8/455170/dd901224bfc1/emboj00150-0348-b.jpg

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1
DNA-dependent RNA polymerase of Escherichia coli induces bending or an increased flexibility of DNA by specific complex formation.
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2
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引用本文的文献

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Trajectory of DNA in the RNA polymerase II transcription preinitiation complex.

本文引用的文献

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Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.从分离的亚基重构细菌DNA依赖性RNA聚合酶作为阐明亚基在转录中作用的工具。
FEBS Lett. 1970 Dec;11(3):165-168. doi: 10.1016/0014-5793(70)80519-1.
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Mobility of DNA in gel electrophoresis.DNA在凝胶电泳中的迁移率。
Biopolymers. 1982 Nov;21(11):2315-6. doi: 10.1002/bip.360211116.
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Why does the electrophoretic mobility of DNA in gels vary with the length of the molecule?为什么DNA在凝胶中的电泳迁移率会随分子长度而变化？
RNA聚合酶II转录起始前复合物中DNA的轨迹。
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12268-73. doi: 10.1073/pnas.94.23.12268.
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DNA sequence determinants of LexA-induced DNA bending.
Nucleic Acids Res. 1993 May 25;21(10):2363-7. doi: 10.1093/nar/21.10.2363.
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The main early and late promoters of Bacillus subtilis phage phi 29 form unstable open complexes with sigma A-RNA polymerase that are stabilized by DNA supercoiling.枯草芽孢杆菌噬菌体 phi 29 的主要早期和晚期启动子与 σA-RNA 聚合酶形成不稳定的开放复合物，这些复合物通过 DNA 超螺旋得以稳定。
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Three-dimensional model of yeast RNA polymerase I determined by electron microscopy of two-dimensional crystals.通过二维晶体的电子显微镜技术确定的酵母RNA聚合酶I的三维模型。
EMBO J. 1993 Jul;12(7):2601-7. doi: 10.1002/j.1460-2075.1993.tb05920.x.
7
Promoters responsive to DNA bending: a common theme in prokaryotic gene expression.对DNA弯曲有反应的启动子：原核生物基因表达中的一个共同主题。
Microbiol Rev. 1994 Jun;58(2):268-90. doi: 10.1128/mr.58.2.268-290.1994.
8
CRP fixes the rotational orientation of covalently closed DNA molecules.CRP固定共价闭合DNA分子的旋转方向。
EMBO J. 1994 Oct 17;13(20):4983-90. doi: 10.1002/j.1460-2075.1994.tb06825.x.
9
Role of CRP in transcription activation at Escherichia coli lac promoter: CRP is dispensable after the formation of open complex.CRP在大肠杆菌乳糖启动子转录激活中的作用：在开放复合物形成后CRP是可有可无的。
Nucleic Acids Res. 1995 Feb 25;23(4):599-605. doi: 10.1093/nar/23.4.599.
10
Translocation of the Escherichia coli transcription complex observed in the registers 11 to 20: "jumping" of RNA polymerase and asymmetric expansion and contraction of the "transcription bubble".在寄存器11至20中观察到的大肠杆菌转录复合物的易位：RNA聚合酶的“跳跃”以及“转录泡”的不对称扩张和收缩。
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1739-43. doi: 10.1073/pnas.92.5.1739.
Biopolymers. 1982 May;21(5):995-7. doi: 10.1002/bip.360210511.
4
Topography of interaction of Escherichia coli RNA polymerase subunits with lac UV5 promoter.大肠杆菌RNA聚合酶亚基与lac UV5启动子的相互作用拓扑结构
FEBS Lett. 1981 Jun 1;128(1):46-50. doi: 10.1016/0014-5793(81)81076-9.
5
Structure of the nucleosome core particle at 7 A resolution.7埃分辨率下核小体核心颗粒的结构。
Nature. 1984;311(5986):532-7. doi: 10.1038/311532a0.
6
The locus of sequence-directed and protein-induced DNA bending.序列导向和蛋白质诱导的DNA弯曲位点。
Nature. 1984;308(5959):509-13. doi: 10.1038/308509a0.
7
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.一种用于定量蛋白质与特定DNA区域结合的凝胶电泳方法：应用于大肠杆菌乳糖操纵子调控系统的组分
Nucleic Acids Res. 1981 Jul 10;9(13):3047-60. doi: 10.1093/nar/9.13.3047.
8
Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase.大肠杆菌RNA聚合酶σ亚基的过表达与纯化
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9
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.一种从大肠杆菌大规模制备高纯度依赖DNA的RNA聚合酶的新方法。
Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):221-4. doi: 10.1515/bchm2.1970.351.1.221.
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DNA bending and its relation to nucleosome positioning.DNA弯曲及其与核小体定位的关系。
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