Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, 77843, USA.
Department of Cell & Molecular Biology, Tulane University, New Orleans, LA, 70118, USA.
Nat Commun. 2019 Feb 5;10(1):424. doi: 10.1038/s41467-018-08278-4.
A major goal of regenerative medicine is to stimulate tissue regeneration after traumatic injury. We previously discovered that treating digit amputation wounds with BMP2 in neonatal mice stimulates endochondral ossification to regenerate the stump bone. Here we show that treating the amputation wound with BMP9 stimulates regeneration of a synovial joint that forms an articulation with the stump bone. Regenerated structures include a skeletal element lined with articular cartilage and a synovial cavity, and we demonstrate that this response requires the Prg4 gene. Combining BMP2 and BMP9 treatments in sequence stimulates the regeneration of bone and joint. These studies provide evidence that treatment of growth factors can be used to engineer a regeneration response from a non-regenerating amputation wound.
再生医学的主要目标是在创伤后刺激组织再生。我们之前发现，在新生小鼠的断指伤处用 BMP2 治疗可刺激软骨内骨化以再生残端骨。在这里，我们表明用 BMP9 治疗断肢伤可刺激形成与残端骨关节连接的滑液关节的再生。再生的结构包括一个由关节软骨衬里的骨骼元素和一个滑液腔，我们证明这种反应需要 Prg4 基因。顺序使用 BMP2 和 BMP9 治疗可刺激骨骼和关节的再生。这些研究为治疗生长因子可用于从不可再生的断肢伤诱导再生反应提供了证据。
