• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

皮下注射血小板衍生生长因子-AA可改善脊髓损伤后的功能恢复。

Subcutaneous Administration of PDGF-AA Improves the Functional Recovery After Spinal Cord Injury.

作者信息

Guo Xue-Yan, Duan Fei-Xiang, Chen Jing, Wang Ying, Wang Rui, Shen Lin, Qi Qi, Jiang Zhi-Quan, Zhu An-You, Xi Jin, Lü He-Zuo, Hu Jian-Guo

机构信息

Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China.

出版信息

Front Neurosci. 2019 Jan 22;13:6. doi: 10.3389/fnins.2019.00006. eCollection 2019.

DOI:10.3389/fnins.2019.00006
PMID:30723394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6349709/
Abstract

Previous studies by our group have demonstrated that the transplantation of exogenous platelet-derived growth factor (PDGF)-AA-overexpressing oligodendrocyte progenitor cells (OPCs) promotes tissue repair and recovery of neurological function in a rat model of spinal cord injury (SCI). However, it remains unclear whether treatment with PDGF-AA also affects endogenous oligodendrocytes (OLs) or even neurons, thus promoting further functional recovery after SCI. In the present study, we evaluated the therapeutic potential of PDGF-AA treatment by direct subcutaneous injection of PDGF-AA immediately after SCI. We demonstrated that PDGF-AA injection resulted in increased tissue sparing, myelination and functional recovery in rats following SCI. Further experimentation confirmed that PDGF-AA increased the survival of endogenous OPCs and OLs, and promoted the proliferation of OPCs and their differentiation into OLs. Moreover, PDGF-AA also protected motor neurons from death in the injured spinal cord. These results indicated that PDGF-AA administration may be an effective treatment for SCI.

摘要

我们团队之前的研究表明，在脊髓损伤（SCI）大鼠模型中，移植外源性过表达血小板衍生生长因子（PDGF）-AA的少突胶质细胞祖细胞（OPC）可促进组织修复和神经功能恢复。然而，尚不清楚PDGF-AA治疗是否也会影响内源性少突胶质细胞（OL）甚至神经元，从而促进SCI后的进一步功能恢复。在本研究中，我们通过在SCI后立即直接皮下注射PDGF-AA来评估其治疗潜力。我们证明，PDGF-AA注射可增加SCI大鼠的组织保留、髓鞘形成和功能恢复。进一步的实验证实，PDGF-AA可提高内源性OPC和OL的存活率，并促进OPC的增殖及其向OL的分化。此外，PDGF-AA还可保护运动神经元免于在受损脊髓中死亡。这些结果表明，给予PDGF-AA可能是治疗SCI的有效方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/016bfca74d3e/fnins-13-00006-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/e595a756abf1/fnins-13-00006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/29f2a0b98c96/fnins-13-00006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/3053fe7abc13/fnins-13-00006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/9e9867cc76aa/fnins-13-00006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/eda9a0afffc5/fnins-13-00006-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/67a2fea727d7/fnins-13-00006-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/2ee83e9a842c/fnins-13-00006-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/016bfca74d3e/fnins-13-00006-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/e595a756abf1/fnins-13-00006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/29f2a0b98c96/fnins-13-00006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/3053fe7abc13/fnins-13-00006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/9e9867cc76aa/fnins-13-00006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/eda9a0afffc5/fnins-13-00006-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/67a2fea727d7/fnins-13-00006-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/2ee83e9a842c/fnins-13-00006-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0865/6349709/016bfca74d3e/fnins-13-00006-g008.jpg

相似文献

1
Subcutaneous Administration of PDGF-AA Improves the Functional Recovery After Spinal Cord Injury.皮下注射血小板衍生生长因子-AA可改善脊髓损伤后的功能恢复。
Front Neurosci. 2019 Jan 22;13:6. doi: 10.3389/fnins.2019.00006. eCollection 2019.
2
Transplantation of PDGF-AA-Overexpressing Oligodendrocyte Precursor Cells Promotes Recovery in Rat Following Spinal Cord Injury.过表达血小板源性生长因子-AA的少突胶质前体细胞移植促进脊髓损伤大鼠的恢复。
Front Cell Neurosci. 2017 Mar 21;11:79. doi: 10.3389/fncel.2017.00079. eCollection 2017.
3
Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury.过表达 MRF 的少突胶质前体细胞和施万细胞共移植促进大鼠脊髓损伤后的恢复。
Neurobiol Dis. 2016 Oct;94:196-204. doi: 10.1016/j.nbd.2016.06.016. Epub 2016 Jun 29.
4
TLR4 Deficiency Impairs Oligodendrocyte Formation in the Injured Spinal Cord.Toll样受体4缺陷损害损伤脊髓中少突胶质细胞的形成。
J Neurosci. 2016 Jun 8;36(23):6352-64. doi: 10.1523/JNEUROSCI.0353-16.2016.
5
Improved differentiation of oligodendrocyte precursor cells and neurological function after spinal cord injury in rats by oscillating field stimulation.振荡场刺激改善大鼠脊髓损伤后少突胶质前体细胞的分化及神经功能
Neuroscience. 2015 Sep 10;303:346-51. doi: 10.1016/j.neuroscience.2015.07.017. Epub 2015 Jul 9.
6
Oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells derived from adult rat spinal cord: in vitro characteristics and response to PDGF, bFGF and NT-3.源自成年大鼠脊髓的少突胶质细胞-2型星形胶质细胞（O-2A）祖细胞：体外特性及对血小板源性生长因子、碱性成纤维细胞生长因子和神经营养因子-3的反应
Glia. 1996 Jan;16(1):16-26. doi: 10.1002/(SICI)1098-1136(199601)16:1<16::AID-GLIA3>3.0.CO;2-9.
7
Transplantation of oligodendrocyte precursor cells improves myelination and promotes functional recovery after spinal cord injury.少突胶质前体细胞移植可改善髓鞘形成，并促进脊髓损伤后的功能恢复。
Injury. 2012 Jun;43(6):794-801. doi: 10.1016/j.injury.2011.09.013. Epub 2011 Oct 20.
8
Platelet-derived growth factor delays oligodendrocyte differentiation and axonal myelination in vivo in the anterior medullary velum of the developing rat.血小板衍生生长因子会延迟发育中大鼠延髓前帆体内少突胶质细胞的分化和轴突髓鞘形成。
J Neurosci Res. 1997 Jun 15;48(6):588-96.
9
Encapsulated oligodendrocyte precursor cell fate is dependent on PDGF-AA release kinetics in a 3D microparticle-hydrogel drug delivery system.在 3D 微颗粒-水凝胶药物输送系统中，包封少突胶质前体细胞的命运依赖于 PDGF-AA 的释放动力学。
J Biomed Mater Res A. 2018 Sep;106(9):2402-2411. doi: 10.1002/jbm.a.36432. Epub 2018 May 14.
10
Chd7 Collaborates with Sox2 to Regulate Activation of Oligodendrocyte Precursor Cells after Spinal Cord Injury.Chd7与Sox2协同调节脊髓损伤后少突胶质前体细胞的激活。
J Neurosci. 2017 Oct 25;37(43):10290-10309. doi: 10.1523/JNEUROSCI.1109-17.2017. Epub 2017 Sep 20.

引用本文的文献

1
Single-cell analysis of innate spinal cord regeneration identifies intersecting modes of neuronal repair.单细胞分析先天脊髓再生鉴定神经元修复的相交模式。
Nat Commun. 2024 Aug 15;15(1):6808. doi: 10.1038/s41467-024-50628-y.
2
Promotive effect of skin precursor-derived Schwann cells on brachial plexus neurotomy and motor neuron damage repair through milieu-regulating secretome.皮肤前体细胞来源的雪旺细胞通过调节微环境的分泌组对臂丛神经切断和运动神经元损伤修复的促进作用。
Regen Ther. 2024 Apr 22;27:365-380. doi: 10.1016/j.reth.2024.04.002. eCollection 2024 Dec.
3
Transplantation of dorsal root ganglia overexpressing the NaChBac sodium channel improves locomotion after complete SCI.

本文引用的文献

1
Traumatic spinal cord injury.外伤性脊髓损伤。
Nat Rev Dis Primers. 2017 Apr 27;3:17018. doi: 10.1038/nrdp.2017.18.
2
Cell transplantation therapy for spinal cord injury.脊髓损伤的细胞移植治疗。
Nat Neurosci. 2017 Apr 25;20(5):637-647. doi: 10.1038/nn.4541.
3
Transplantation of PDGF-AA-Overexpressing Oligodendrocyte Precursor Cells Promotes Recovery in Rat Following Spinal Cord Injury.过表达血小板源性生长因子-AA的少突胶质前体细胞移植促进脊髓损伤大鼠的恢复。
过表达 NaChBac 钠离子通道的背根神经节移植可改善完全性 SCI 后的运动功能。
Mol Ther. 2024 Jun 5;32(6):1739-1759. doi: 10.1016/j.ymthe.2024.03.038. Epub 2024 Mar 30.
4
[Acetylcorynoline inhibits microglia activation by regulating EGFR/MAPK signaling to promote functional recovery of injured mouse spinal cord].乙酰紫堇灵通过调节表皮生长因子受体/丝裂原活化蛋白激酶信号通路抑制小胶质细胞活化，促进脊髓损伤小鼠的功能恢复
Nan Fang Yi Ke Da Xue Xue Bao. 2023 Jun 20;43(6):915-923. doi: 10.12122/j.issn.1673-4254.2023.06.06.
5
Macrophages Treated with VEGF and PDGF Exert Paracrine Effects on Olfactory Ensheathing Cell Function.血管内皮生长因子和血小板衍生生长因子处理的巨噬细胞对嗅鞘细胞功能发挥旁分泌效应。
Cells. 2022 Aug 4;11(15):2408. doi: 10.3390/cells11152408.
6
Molecular approaches for spinal cord injury treatment.脊髓损伤治疗的分子方法。
Neural Regen Res. 2023 Jan;18(1):23-30. doi: 10.4103/1673-5374.344830.
7
Towards Secretome Standardization: Identifying Key Ingredients of MSC-Derived Therapeutic Cocktail.迈向分泌组标准化：鉴定间充质干细胞衍生治疗性鸡尾酒的关键成分。
Stem Cells Int. 2021 Aug 26;2021:3086122. doi: 10.1155/2021/3086122. eCollection 2021.
8
Neuroprotective effects of P7C3 against spinal cord injury in rats.P7C3对大鼠脊髓损伤的神经保护作用。
Exp Biol Med (Maywood). 2019 Dec;244(18):1680-1687. doi: 10.1177/1535370219888620. Epub 2019 Nov 13.
Front Cell Neurosci. 2017 Mar 21;11:79. doi: 10.3389/fncel.2017.00079. eCollection 2017.
4
Traumatic spinal cord injury in the United States, 1993-2012.1993 - 2012年美国创伤性脊髓损伤情况
JAMA. 2015 Jun 9;313(22):2236-43. doi: 10.1001/jama.2015.6250.
5
Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2.施万细胞通过分泌血小板源性生长因子-AA（PDGF-AA）和碱性成纤维细胞生长因子（FGF-2）诱导少突胶质前体细胞增殖和迁移。
J Mol Neurosci. 2015 Aug;56(4):999-1008. doi: 10.1007/s12031-015-0570-1. Epub 2015 Jun 5.
6
Translating mechanisms of neuroprotection, regeneration, and repair to treatment of spinal cord injury.将神经保护、再生和修复机制转化应用于脊髓损伤的治疗
Prog Brain Res. 2015;218:15-54. doi: 10.1016/bs.pbr.2014.12.007. Epub 2015 Mar 29.
7
Role of endogenous neural stem cells in spinal cord injury and repair.内源性神经干细胞在脊髓损伤与修复中的作用。
JAMA Neurol. 2015 Feb;72(2):235-7. doi: 10.1001/jamaneurol.2014.2927.
8
PDGF suppresses oxidative stress induced Ca2+ overload and calpain activation in neurons.血小板衍生生长因子可抑制神经元氧化应激诱导的钙离子超载和钙蛋白酶激活。
Oxid Med Cell Longev. 2013;2013:367206. doi: 10.1155/2013/367206. Epub 2013 Dec 24.
9
From basics to clinical: a comprehensive review on spinal cord injury.从基础到临床：脊髓损伤的全面综述。
Prog Neurobiol. 2014 Mar;114:25-57. doi: 10.1016/j.pneurobio.2013.11.002. Epub 2013 Nov 20.
10
Cotransplantation of glial restricted precursor cells and Schwann cells promotes functional recovery after spinal cord injury.胶质限制前体细胞和许旺细胞共移植促进脊髓损伤后的功能恢复。
Cell Transplant. 2013;22(12):2219-36. doi: 10.3727/096368912X661373. Epub 2013 Jan 2.