食蟹猴感染结核分枝杆菌期间的CD4CD8双阳性T细胞反应
CD4CD8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques.
作者信息
Diedrich Collin Richard, Gideon Hannah Priyadarshini, Rutledge Tara, Baranowski Tonilynn Marie, Maiello Pauline, Myers Amy J, Lin Philana Ling
机构信息
Department of Pediatrics, Children's Hospital of Pittsburgh of the University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
出版信息
J Med Primatol. 2019 Apr;48(2):82-89. doi: 10.1111/jmp.12399. Epub 2019 Feb 5.
BACKGROUND
Tuberculosis (TB) kills millions of people every year. CD4 and CD8 T cells are critical in the immune response against TB. T cells expressing both CD4 and CD8 (CD4CD8 T cells) are functionally active and have not been examined in the context of TB.
METHODS
We examine peripheral blood mononuclear cells (PBMC) and bronchoalveolar lavage cells (BAL) and lung granulomas from 28 cynomolgus macaques during Mycobacterium tuberculosis (Mtb) infection.
RESULTS
CD4CD8 T cells increase in frequency during early Mtb infection in PBMC and BAL from pre-infection. Peripheral, airway, and lung granuloma CD4CD8 T cells have distinct patterns and greater cytokine production than CD4 or CD8 T cells.
CONCLUSION
Our data suggest that CD4CD8 T cells transient the blood and airways early during infection to reach the granulomas where they are involved directly in the host response to Mtb.
背景
结核病每年导致数百万人死亡。CD4和CD8 T细胞在抗结核免疫反应中至关重要。同时表达CD4和CD8的T细胞（CD4CD8 T细胞）具有功能活性，尚未在结核病背景下进行研究。
方法
我们检测了28只食蟹猕猴在感染结核分枝杆菌（Mtb）期间的外周血单个核细胞（PBMC）、支气管肺泡灌洗细胞（BAL）和肺肉芽肿。
结果
在Mtb感染早期，PBMC和BAL中CD4CD8 T细胞的频率从感染前开始增加。外周、气道和肺肉芽肿中的CD4CD8 T细胞具有不同的模式，并且比CD4或CD8 T细胞产生更多的细胞因子。
结论
我们的数据表明，CD4CD8 T细胞在感染早期短暂出现在血液和气道中，以到达肉芽肿，在那里它们直接参与宿主对Mtb的反应。
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