在感染结核分枝杆菌期间，食蟹猴血液和气道中的自然杀伤细胞的特征。
Characterization of natural killer cells in the blood and airways of cynomolgus macaques during Mycobacterium tuberculosis infection.
机构信息
Department of Pediatrics, Children's Hospital of Pittsburgh of the University of Pittsburgh Medical Center, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
出版信息
J Med Primatol. 2023 Feb;52(1):24-33. doi: 10.1111/jmp.12617. Epub 2022 Sep 3.
BACKGROUND
Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb) and kills more than 1.5 million people each year.
METHODS
We examine the frequency and function of NK cells in the blood and airways over the course of Mtb infection in a TB macaque model and demonstrate differences in NK marker expression between the two compartments. Flow cytometry and intracellular cytokine staining were utilized to identify NK cell subsets (expressing NKG2A, CD56, or CD16) and function (IL-10, TNF, IL-2, IFN-g, IL-17, and CD107a).
RESULTS
Blood and airway NK cell frequencies were similar during infection though there were differences in subset populations between blood and airway. Increased functional (cytokine/CD107a) parameters were observed in airway NK cells during the course of infection while none were seen in the blood.
CONCLUSIONS
This study suggests that NK cells in the airway may play an important role in TB host response.
背景
结核病（TB）是由结核分枝杆菌（Mtb）引起的，每年导致超过 150 万人死亡。
方法
我们在结核猕猴模型中检查了 Mtb 感染过程中血液和气道中 NK 细胞的频率和功能，并证明了两个部位之间 NK 标志物表达的差异。流式细胞术和细胞内细胞因子染色用于鉴定 NK 细胞亚群（表达 NKG2A、CD56 或 CD16）和功能（IL-10、TNF、IL-2、IFN-g、IL-17 和 CD107a）。
结果
尽管血液和气道中的亚群存在差异，但在感染过程中血液和气道中的 NK 细胞频率相似。在感染过程中，气道 NK 细胞的功能（细胞因子/CD107a）参数增加，而血液中则没有观察到。
结论
这项研究表明，气道中的 NK 细胞可能在 TB 宿主反应中发挥重要作用。
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